Screening the Libraries Using Various Single Locus Markers
| Chromosome 5 | Chromosome 19 | ||||||
| Markers | Locus | No. of positives | Markers | Locus | No. of positives | ||
| Segment 1–4 | Segment 5 | Segment 1–4 | Segment 5 | ||||
| D5S417 | 5.5 cM | 27 | 11 | D19S221 | 35.5 cM | 22 | 2 |
| D5S406 | 10.7 cM | 26 | 9 | D19S411 | 31 | 10 | |
| D5S635 | 13.8 cM | 26 | 7 | D19S425 | 58.7 cM | 21 | 9 |
| D5S676 | 15.6 cM | 28 | 7 | D19S220 | 61.4 cM | 18 | 2 |
| D5S1986 | 44.5 cM | 37 | 3 | D19S422 | 62.5 cM | 14 | 8 |
| D5S426 | 51.6 cM | 25 | 5 | D19S219 | 69.9 cM | 21 | 3 |
| D5S634 | 59.9 cM | 34 | 5 | D19S412 | 69.9 cM | 22 | 5 |
| D5S2076 | 62.5 cM | 21 | 5 | D19S596 | 77.6 cM | 16 | 6 |
| D5S407 | 65.0 cM | 24 | 13 | D19S214 | 106.1 cM | 21 | 8 |
| D5S491 | 67.2 cM | 25 | 8 | ||||
| D5S2028 | 69.0 cM | 31 | 5 | Chromosome 21 | |||
| D5S2029 | 92.5 cM | 25 | 10 | Markers | No. of positives | ||
| D5S456 | 109.3 cM | 24 | 2 | D21S1904 | 0.0 cM | 23 | 7 |
| D5S505 | 111.6 cM | 24 | 2 | D21S1911 | 0.1 cM | 28 | 3 |
| D5S2065 | 121.7 cM | 20 | 10 | D21S262 | 32.6 cM | 26 | 6 |
| D5S657 | 130.1 cM | 27 | 4 | D21S1252 | 38.7 cM | 13 | 2 |
| D5S2017 | 144.8 cM | 23 | 7 | D21S1893 | 48.1 cM | 27 | 10 |
| D5S2090 | 149.9 cM | 31 | 5 | D21S1260 | 51.6 cM | 22 | 3 |
| D5S673 | 155.5 cM | 16 | 2 | ||||
| D5S487 | 157.6 cM | 18 | 8 | Average number | |||
| D5S412 | 161.0 cM | 29 | 5 | 23.9 | 6.0 | ||
| D5S403 | 162.2 cM | 15 | 13 | ||||
| D5S2066 | 164.9 cM | 17 | 4 | Standard deviation | |||
| D5S2032 | 168.5 cM | 46 | 9 | 6.56 | 3.04 | ||
| D5S672 | 85.5 cM | 24 | 3 | ||||
| D5S413 | 150.0 cM | 15 | 2 | ||||
| D5S496 | 172.6 cM | 24 | 6 | ||||
| D5S2030 | 190.9 cM | 12 | 7 | ||||
| D5S1987 | 21.9 cM | 31 | 5 | ||||
| D5S1991 | 25.9 cM | 26 | 6 | ||||
[i] A total of 45 markers (30 from chromosome 5, 9 from chromosome 19, and 6 from chromosome 21) have been used to screen the RPCI-11 library. The average genome representation of EcoRI andMboI segments was determined to be 23.9 and 6.0 based on the screening results. Standard deviation from the average number of positive clones for EcoRI and MboI segments were estimated to be 6.56 and 3.04, respectively. Overall genome redundancy has been estimated at 23.1-fold for EcoRI library part and 6.3-fold for MboI based on the average insert size and the number of clones. The locus column indicates the human chromosome location from the top of each chromosome linkage group.