Table 3.

Genes Showing Major Expression Changes after Addition of 125 ng/mL of Novobiocin

No.[i] Gene product Fold change mRNA (protein)
10 min 30 min 60 min
hi0944Riboflavin biosynthesis protein2.92.93.6
hi0873UDP-N-acetylglucosamine epimerase4.64.73.4
hi1722Methionine aminopeptidase3.5 (NC)3.9 (NC)3.4 (3.2)
hi1051Leukotoxin secretion ATP-binding protein3.13.93.3
hi1623Mercury resistance regulatory protein3.22.73.2
hi0991DNA/ATP binding protein2.52.73.1
hi0872Undecaprenyl-phosphate galactosephosphotransferase3.64.33.0
hi1305Hydrogenase gene region2.73.22.9
hi1573Pyruvate kinase type II2.3 (NC)2.3 (NC)2.7 (NC)
hi0394Peptidyl-tRNA hydrolase2.62.42.6
hi0533RNA polymerase sigma-70 factor2.42.12.6
hi1247Excinuclease ABC subunit B2.22.42.5
hi1304N utilization substance protein B2.32.92.5
hi1441Stringent starvation protein A2.3 (NC)2.9 (2.2)2.5 (NC)
hi0567DNA gyrase, subunit B2.12.92.4
hi0603Uroporphyrinogen III methylase2.72.12.4
hi1307Virulence plasmid protein2.42.52.3
hi0769Cell division ATP-binding protein3.02.52.3
hi09703-Dehydroquinase2.32.22.2
hi1264DNA gyrase, subunit A2.52.12.2
hi0992DNA polymerase III beta-subunit2.2 (NC)2.5 (NC)2.2 (NC)
hi0969O-Succinylbenzoate-CoA synthase2.12.42.2
hi0623Methionyl-tRNA formyltransferase3.02.82.1
hi0625TRK system potassium uptake protein3.13.02.1
hi0236ArsC protein−2.4−2.3−2.1
hi1734Enoyl-reductase[ii] −2.6 (−8.8)−2.6 (−7.2)−2.1 (−6.2)
hi0422ATP-dependent RNA helicase−2.8−2.6−2.1
hi0537Urease accessory protein−2.3−4.0−2.1
hi1661Dihydrolipoamide succinyltransferase−4.7 (NC)−2.6 (NC)−2.2 (NC)
hi0425Phosphatidylserine synthase−2.9−2.1−2.2
hi0863Pyridoxamine phosphate oxidase−3.0−3.4−2.2
hi1434.1Cold shock-like protein−3.0−2.1−2.2
hi1186Dipeptide transport system permease protein−2.5−2.5−2.2
hi1676Molybdenum cofactor biosynthesis protein A−3.9−3.5−2.2
hi1040Restriction enzyme−3.0−3.4−2.2
hi0503High affinity ribose transport protein−3.4−2.9−2.3
hi1152PmbA protein homolog−3.0−2.2−2.3
hi1193Branched-chain-amino-acid transaminase−2.3 (NC)−2.5 (−1.5)−2.3 (NC)
hi0538Urease accessory protein−2.9−3.2−2.3
hi1033Phosphoserine phosphatase−5.0−2.8−2.3
hi1632Lysine-sensitive aspartokinase III−3.3−2.4−2.3
hi1227Uracil permease−2.3−2.8−2.4
hi1041Modification methylase HgiDI−2.8−2.4−2.5
hi1163D-Lactate dehydrogenase−3.2−3.2−2.5
hi0117Membrane-bound lytic murein transglycosylase A−2.5−2.8−2.6
hi1022Biotin synthetase−4.7−3.4−2.6
hi0563Regulatory protein AsnC−2.7−2.5−2.6
hi0539Urease, alpha subunit−3.2 (NC)−3.8 (NC)−2.6 (NC)
hi1201Adenine specific methylase, putative−2.7−2.9−2.7
hi1682Putative protease−2.9−2.3−2.7
hi1065MukB suppressor protein−2.5−2.5−2.9
hi1545C4-Dicarboxylate transport protein−5.0−3.9−2.9
hi1383Periplasmic phosphate-binding protein−2.1−4.0−3.0
hi0078Cys-tRNA synthetase−2.6−2.5−3.0
hi1263Homoserine acetyltransferase−2.7−2.6−3.1
hi0419Collagenase−4.3−3.2−3.2
hi1106Xylose operon regulatory protein−2.8−2.7−3.2
hi1507Hypothetical proteinNC (−4.1)−3.5 (−3.2)−3.2 (−2.9)
hi1068Formate-dependent nitrite reductase−3.0−2.7−3.3
hi1735Peptide-chain-release factor 3−4.3−3.3−3.4
hi1112Xylose isomerase−2.3−3.2−3.4
hi1019Thiamin-binding periplasmic protein−5.0−4.7−3.4
hi0445Protein translocation protein, low temperature−3.6−4.6−3.8
hi0548Initiation factor IF-1−7.7−5.8−4.0
hi1181Phosphoheptose isomerase−4.2−5.2−4.0
hi1172S-Adenosylmethionine synthetase 2[iii] −3.9 (NC)−4.1 (3.0)−4.0 (NC)
hi1166Histidinol-phosphate aminotransferase−6.5−4.1−4.1
hi1171Anthranilate synthase component II−3.8−3.4−4.1
hi0501High affinity ribose transport protein−2.7−2.4−4.1
hi0182Glucose kinase−3.1−5.2−4.3
hi1728Branched chain amino acid transport  system II carrier protein−9.5−7.5−4.5
hi1170Anthranilate synthase component I−5.7−4.2−4.8
hi054916S rRNA-dimethyltransferase−7.6−5.0−4.9
hi0808Ribosome releasing factor−4.5 (−4.7)−5.3 (−4.4)−4.9 (−4.7)
hi0214Oligopeptidase A−4.8−6.8−5.0
hi1009Glycerol-3-phosphate regulon repressor−7.4−9.8−5.0
hi0179Pyruvate formate-lyase activating enzyme−6.9−6.1−5.1
hi0211Phosphatidylglycerophosphatase B−4.5−7.6−5.7
hi1365Topoisomerase I−4.4−5.8−6.1
hi1167Phosphoserine aminotransferase−9.1−9.6−6.2
hi1154Proton glutamate symport protein−4.2−4.1−6.3
hi1729Lactam utilization protein−6.9−9.3−6.5
hi1048Hypothetical protein−9.1−14.1−9.6
hi1035Magnesium and cobalt transport protein−9.2−10.5−12.2

[i] Numbering according to Fleischmann et al. (1995).

[ii] Present as two spots; other spot decreased to a lesser extent.

[iii] Present as two spots; other spot increased in intensity at the 30-min time point.