Table 3.
Most Significant Hexamers in 3′ Fragments: Scattered Hexamers
| Hexamer | Observed (expected)[i] | % sites | P [ii] | Position average ± SD | Location[iii] | ||
% sites
P
Position average ± SD
Location
62 (10)1.19 × 10−28
−19 ± 11
49 (10)0.84 × 10−18
−20 ± 1069 (20)1.23 × 10−18
−17 ± 1229 (5)0.58 × 10−12
−20 ± 1022 (3)0.39 × 10−12
−24 ± 13/embed/graphic-3.gif)
| 62 (10) | 1.1 | 9 × 10−28 | −19 ± 11 |
% sites
P
Position average ± SD
Location
62 (10)1.19 × 10−28
−19 ± 11
49 (10)0.84 × 10−18
−20 ± 1069 (20)1.23 × 10−18
−17 ± 1229 (5)0.58 × 10−12
−20 ± 1022 (3)0.39 × 10−12
−24 ± 13/embed/graphic-4.gif)
| ||
| 49 (10) | 0.8 | 4 × 10−18 | −20 ± 10 | ||||
| 69 (20) | 1.2 | 3 × 10−18 | −17 ± 12 | ||||
| 29 (5) | 0.5 | 8 × 10−12 | −20 ± 10 | ||||
| 22 (3) | 0.3 | 9 × 10−12 | −24 ± 13 | ||||
[i] Expected occurrences based on a random distribution in a database of same nucleotide composition.
[ii] Probability of reaching the observed frequency by chance based on a cumulative binomial distribution. All sequences containing the most significant hexamer were removed from the database before the next most significant hexamer was sought.
[iii] Location of the last base of hexamer. Position 0 is the putative poly(A) site.