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Table 3.

Primer Sequences

Primer (exon)[i]

Sequence (5′-3′)

Length

T _m (°C)

Product (bp)

1, F

A

G

C

C

T

G

G

G

T

C

G

G

T

T

G

T

A

C

G

19

1, R

A

G

C

A

G

G

T

G

T

T

C

C

T

C

G

G

G

A

G

19

60

684

2, F

A

T

T

G

T

G

T

C

T

T

C

A

T

G

A

C

T

T

C

19

2, R

G

A

A

T

T

C

T

T

C

C

T

C

A

T

T

C

A

C

18

50

953

3a, F

A

G

C

T

G

T

T

G

T

G

A

T

C

A

T

T

G

T

C

19

3a, R

G

A

G

T

T

G

T

G

G

T

G

G

T

T

G

A

A

G

18

54

1122

3b, F

T

C

C

C

A

C

G

A

C

T

A

C

C

A

C

C

A

A

G

19

3b, R

A

C

C

G

G

T

C

T

C

C

A

C

A

G

A

C

A

A

C

19

55

1507

3c, F

G

C

C

G

G

C

A

C

C

T

C

C

A

G

T

T

T

A

T

C

C

21

3c, R

G

T

C

A

T

G

T

T

C

T

T

G

G

G

A

C

G

G

G

A

G

C

22

68

514

3d, F

C

C

T

T

T

A

C

G

G

C

C

C

C

C

A

T

G

G

A

G

20

3d, R

C

G

C

C

A

C

C

A

G

A

C

C

T

T

T

C

C

C

A

G

20

65

1296

3e, F

T

C

C

C

A

A

C

A

G

T

C

C

A

C

A

G

C

C

C

19

3e, R

T

C

C

C

C

T

C

T

T

C

C

C

T

C

T

G

T

C

A

C

A

21

61

1402

[i] (F) Forward; (R) reverse.