Table 3.

Expression Analysis of Candidate Sequences by Northern Hybridization and RT–PCR

Sequence name Clone ID[i] Sequence available (kb) Northern RT–PCR
band size (kb) tissue specificity[ii] MB231 PaCa-2 ZR-75 SW480
R80652 146794 (3′)1.86[iii] noneN.A.++
20B08e10.1[iii] 1.2all tissues tested
20B08e40.5[iii] 1.2all tissues tested++++
20B08e50.35[iii] noneN.A.++++
D78237 0.154noneN.A.+
R26196 133050 (3′)1.22[iii] noneN.A.++

[i] IMAGE Consortium clone ID. Clones are available through Research Genetics.

[ii] Tissues used for analysis are: spleen, thymus, prostate, testis, ovary, small intestine, colon, PBL. (N.A.) Not applicable.

[iii] Available sequence information for clones R80652, 20B08e1, 20B08e4, 20B08e5 and R26196 was obtained upon sequencing of the original clones and Rapid Amplification of cDNA Ends (RACE) products.