Amplimers Derived from Direct Sequencing of PAC, BAC, or Cosmid Ends
| STS | P1 (5′ → 3′) | P2 (5′ → 3′) | STS size (bp) |
| 141B3-T7 | GGGGGTTTGGTGTTATTGAC | ATCCTTCCTGATGGCAACTA | 246 |
| 141B3-SP6 | TGTCAGGTGTGTACCTGTGA | CAGCATACCTACTTAAGGGG | 150 |
| 128M19-SP6 | GTGAGGTCCTGAATCATCC | ACTCCCAAGAGCTGAAGGT | 160 |
| 70I24-T7 | GGGTAAGCTCGTTTCTGG | AGGGAGAGATGCTAGAAGC | 230 |
| 70I24-SP6 | AGGAACTAGTGCTCAGCTGG | GCACTCTAAGACTGCCACC | 235 |
| B291B3R | CCAACAACCATCGGTTAATCTTCT | TAATAGAGATGTGACTACAGATTG | 165 |
| B291B3L | TGACATGCATAACTCACGA | CTCATTGTAATACCTTCGC | 117 |
| 171F15-T7 | GGAGTACCATTAGGTGTTTTC | CTGCACATAATTATAAATCG | 255 |
| 269A14-SP6 | GGCTAGGAGGCAGCATCG | TAATACCCTTGGGACGGG | 339 |
| Q14C10- T3 | TGCTGCTCACTCAGCATG | ACAAAGGTCTTGAGCGCC | 170 |
| Q14C10- T7 | GAGTTCAGGCCTGGGACG | TCTTACCCTCTGATATGCGG | 320 |
| Q87D5-T7 | GGATTTGGAGCCATTGCC | AACCTCCACAGAACCGCC | 251 |
| Q87D5-T3 | GGTTTCTTGGCTGTGTGGTT | ATACTTCTGTGTGACAACCG | 146 |