Ability of S. pneumoniae Mutants Inactivated in Genes of the raf Cluster to Ferment (Acid Production) Raffinose, other Sugars and to Induce α-Galactosidase
| Gene inactivated | Raffinose fermentation | Sugar fermentation[i] | α-Galactosidase activity (U/mg protein)[ii] |
| none | + | all | 228 ± 38 |
| aga | − | all | 0.7 ± 0.1 |
| rafE | − | all | 0.9 ± 0.1 |
| rafF | − | all | 0.8 ± 0.1 |
| rafG | − | all | 0.7 ± 0.1 |
| gtfA | + | all | 219 ± 25 |
| rafX | − | all | 0.7 ± 0.1 |
[i] Glucose, fructose, sucrose, galactose, lactose, maltose, inulin, and trehalose.
[ii] The α-galactosidase activities are expressed in units (nmoles of p-nitrophenol produced per min of incubation at room temperature) per mg protein with their s.d.