[i] The chromosomal location of both the target and query sequences are shown. Sequences were aligned using GAP optimal global alignment software. Alignments were analyzed using locally developed software (alignscorer) to determine the number of nucleotide sites compared (L), number of sequence mismatches (m) and the number of insertion/deletions (INDEL). The number of substitutions per nucleotide site and standard error were calculated using Kimura's-two step method (Kimura 1980). An evolutionary rate of nucleotide substitution for pseudogene sequences (2.2 × 10⁻⁹ substitutions per site per year) was used to approximate the evolutionary age of each duplication.