Table 2.

PCR Primers, Product Sizes, and Annealing Temperatures for Multicopy Amplicons and STSs Outside This Region of Chromosome 6p21.3–p22

STS nameSize (bp)Ann. temp.[i] Primer 1Primer 2
gr

[i] Annealing temperatures indicated are for PTC 220/225 thermocyclers. Optimal annealing temperatures in PE9600 thermocyclers are 5°C higher than indicated (see Methods).