Calibration of Peak Positions of Normal DNA
| Sense strand | Antisense strand | ||||||
| raw[ii] | calibrated using | raw[ii] | calibrated using | ||||
| M[iii] | M + N[iv] | M[iii] | M + N[iv] | ||||
| 6393[v] | 6393.00 | 6393.00 | 6557[v] | 6557.00 | 6557.00 | ||
| 6474 | 6389.01 | 6393.03 | 6638 | 6556.32 | 6557.02 | ||
| 6457 | 6392.16 | 6392.98 | 6623 | 6560.09 | 6557.02 | ||
| 6476 | 6391.79 | 6393.01 | 6641 | 6560.12 | 6557.02 | ||
| 6469 | 6389.53 | 6392.01 | 6635 | 6557.35 | 6557.01 | ||
| average | 6391.10 | 6392.81 | 6558.18 | 6557.01 | |||
| s.d. [vi] | 1.55 | 0.40 | 1.61 | 0.01 | |||
[i] Data points of peaks of R6G-labeled sense strand and R110-labeled antisense strand of PCR product of gyrA wild type examined by five successive runs.
[ii] Raw data without calibration.
[iii] Calibrated using size marker alone.
[iv] Calibrated using size marker and TAMRA-labeled normal PCR products.
[v] Used as a template for calibration.
[vi] Standard deviation.