[i] Data from Cadoret et al. (2008) and Karnani et al. (2010) were analyzed for overlaps as described in the Supplemental Methods. (A,B) Comparisons of overlaps between bubble signals and nascent strand calls from Study 1 (Cadoret et al. 2008) or Study 2 (Karnani et al. 2010). (C) Overlaps between shared nascent strand calls and bubble-containing fragments. (D) Cartoon of overlap possibilities: the orange arrow shows an overlap between nascent strand calls from studies 1 and 2; the red arrows illustrate one or two nascent strands contained within a bubble-containing fragment; the green arrow illustrates an overlap between a single nascent strand and two bubbles; the blue arrow shows concordance among the two nascent strand signals and the bubble containing fragments.