Table 2.

RT-PCR Analysis of 10p11 Genes and ESTs

Position in (contig1), contig2 GenBank acc. no/unigene cluster Expression
K L B H T Li FL FB FH FK
(30269-30668) AL045218 −−−−−−−−+−
133098-132535 AW499533 +++−+−−−+−
351871-365253 AA927631/Hs340030 −−−−−−++−+
382137-382486 AA680406/Hs126913 −−−−−−−−−−
385502-385916 AW301129/Hs318978 +−+−+−++−+
435627-406440 ZNF37A ++++++++++
519524-469848 ZNF33A ++++++++++
553075-580039 ZNF25 ++++++++++
672343-700930 ZNF248 ++++++++++
700012-700440HS562273−−−−−−−−−−
754102-754795 BE378519 +−+−++−−−−
785464-785056 AI248257/Hs149302 +−+−++−−+−
785930-785515 AI991440 +−+−++−−−−
1062408-1062674 AA744917 −−−−−−−−+−
1073813-1074078 BE064203 −−−−−−−−−−
1075595-1075848 BE063630 +−−−+−−−−−
1076792-1076582 AI393188 −−−−−−−−−−
1099274-1099737 AA921809/Hs132449 −−−−+−−−−−
1100666-1101084 BE063346 −−−−−−−−−−

[i] + expression, − no expression. The cDNAs were derived from the following tissues: K—kidney, L—lung, B—brain, H—heart; T—testis, Li—Liver, FL—fetal lung, FB—fetal brain; FH—fetal heart, FK—fetal kidney. We have confirmed that all transcripts are derived from chromosome 10 by using the appropriate RT-PCR primers to analyse a monochromosomal somatic cell hybrid panel (not shown). Chromosomal origin was not confirmed for ESTs/EST clusters where expression was not analysed by RT-PCR. Primer sequences can be found at http://www.ncl.ac.uk/ihg/10p11.htm.