Table 2.

List of Strains

E. colistrains Pertinent genotype
DH10B[i] F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL
JW366DH10B, with pJW349 (trfA203) integrated at the attB site; see Figs. 2 and 3
JW371JW366 [pBAC/oriV]
JW378JW366 [pBAC/oriV+20-kb insert (pJW378)]
JW389JW366 [pBAC/oriV+40-kb insert (p67)]
JW390JW366 [pBAC/oriV+80-kb insert (p69)]
JW427DH10B with pJW424 (trfA254) integrated at the attB site; see Table 1 and Fig. 2
JW439JW427 [pBAC/oriV+108-kb insert (pCG275)]
JW450JW427 [pBAC/oriV+122-kb insert (pCG274)]
JW461DH10B with pJW458 (trfA173) integrated at the attB site; see Table 1and Fig. 2
JW462DH10B with pJW459 (trfA171) integrated at the attB site; see Table 1 and Fig. 2
JW463DH10B with pJW457 (trfA250) integrated at the attB site; see Table 1 and Fig. 2
JW467JW461 [pJW378]
JW468JW462 [pJW378]
JW471JW461 [pBAC/oriV+140-kb insert (pCG273)]
JW472JW462 [pBAC/oriV+140-kb insert (pCG273)]
JW480[ii] GenHogs (Invitrogen) with pJW424 (trfA254) integrated at the attB site, see Table 1and Fig. 2
JW499JW461 [pBAC/oriV+108-kb insert (pCG275)]
JW500JW462 [pBAC/oriV+108-kb insert (pCG275)]
JW501JW463 [pBAC/oriV+108-kb insert (pCG275)]
JW526[iii] Stbl2 cells (Life Technologies/Invitrogen) with pJW424 (trfA254) integrated at the attB site; see Table 1 and Fig.2
JW550JW463[pIndigoBAC/oriV]

[i] The E. coli DH10B strain is described in Invitrogen Catalog 2002, p. 229.

[ii] The GeneHogs strain is described in Invitrogen Catalog 2002, p. 230.

[iii] The Stbl2 strain is the gal+lon− parental strain of Stbl4 described in Invitrogen Catalog 2002, p. 234.

[iv] Brackets indicate plasmid, as carried in the host strain. Plasmid designations (see Table 1) or mutant numbers are in parentheses. Δ( ), deletion (followed by deleted genes, in parentheses, and number).