Table 2.

Characterization of Nine Insertion Mutants of PVA Isolated from the Mutant cDNA Library

Position of insertion Propagation in protoplasts
Region nt En masse selection Inoculation individually Systemic infection in tobacco plants (ng/g)[ii] Plasmid name
P1235nt+5300 (527) pM14
HC-Pro1831++0pM65
P33249+−ntpM9
P33379++5500 (1233)pM45
CI4820+−ntpM60
VPg5956nt−ntpM25
NIa-Pro6595+−ntpM56
NIb8330+−ntpM34
CP9149+−ntpM49
pPVAno insertionni+6150 (375) pM52

[i] nt, not tested because the mutant was probably represented in low numbers in the pPVA-Mu mutant library and was not detected by footprinting analysis prior or after en masse selection in protoplasts; ni, not included in the en masse selection experiment; +, propagation observed in protoplasts; −, no detectable propagation in protoplasts.

[ii] mean amount of PVA coat protein antigen (in nanogram) per gram (g) of infected leaf (SE) tissue as determined by DAS-ELISA using known amounts of PVA virions as a comparison; nt, not tested.