Sulfite Tolerance of Yeast Strains Exhibiting Different Numbers of Repeats of a 76-bp Region in the RecombinantSSU1 Promoter
| Strain | Number of 76-bp repeats in recombinant SSU1promoter | Sulfite concentration (mM) | ||||
| 0 | 1 | 3 | 6 | 8 | ||
| S288c | 0 (nonrecombinant control) | + | + | − | − | − |
| T73 | 4 | + | + | + | − | − |
| CECT10233 | 6 | + | + | + | +/− | − |
| CECT10557 | 3 × 2[ii] | + | + | + | +/− | − |
| Y-9 | 4 × 2[ii] | + | + | + | + | − |
[i] Sulfite sensitivity was determined on YPD+TA plates containing 0–8 mM Na2SO3 as described in Methods. +, growth; −, no growth; +/−, poor growth scored after 24 h. Similar results were obtained in liquid medium in microtiter plates after 40 h.
[ii] These strains are homozygous for the recombinantSSU1 promoter.