[i] Total RNA of 10 different rat tissues (bladder, eye, heart, kidney, large intestine, small intestine, liver, pancreas, placenta, and testis) were extracted, labeled, and hybridized to the Rat1600 chip using standard and identical procedures. Individual feature hybridization data were collected and normalized as described in Methods. Potential splice variants were predicted by SPLICE and NEIGHBORHOOD algorithm. The number of predictions for each tissue type was calculated separately. The selection ratio (R) was set at 10 and other default cutoff values were applied.