Classification of Cloned and Candidate DrosophilaNeuropeptide Receptors by BLAST and Phylogenetic Analysis
| Gene | F/G | Best BLAST hit(s) | Phylogenetic analysis full tree neighbors | Bootstrap | |||||
| Diagnostic | P‖ | fly | P‖ | Worm | P | ||||
| CG6857 | A/III-B | CCKR | 35 | CG6881 | 38 | GASR, CCKR | >900 | ||
| CG6881 | A/III-B | GASR | 45 | CG6857 | 71 | GASR, CCKR | >900 | ||
| CG5811 | A/III-B | GRL106 | 58 | CG10626 | 48 | GRL106 | >700 | ||
| CG10626 | A/III-B | LKR | 77 | CG5811 | 38 | LKR, LSR | >990 | ||
| Takr86C | A/III-B | NK3R[ii] | 65 | Takr99D | 72 | NKR | >500 | ||
| Takr99D | A/III-B | NK3R | 64 | Takr86C | 67 | NKR | >500 | ||
| CG10823 | A/III-B | NFFR | 45 | CG5811 | 43 | C50F7.1 | 38 | OXR | <300 |
| BG:BACR48G21.1 | A/III-B | NK2R | 20 | Takr86C | 21 | NMBR/GRPR/BR | <100 | ||
| CG1147 | A/III-B | NY2R | 31 | C25G6.5 | 33 | NPYR | <500 | ||
| CG7395 | A/III-B | NY2R | 40 | F41E7.3 | 32 | NY2R | <500 | ||
| CG12610 | A/III-B | NPYR | 14 | PRPR | >500 | ||||
| CG13995 | A/III | GASR | 6 | NMBR/GRPR/BR | <100 | ||||
| CG14593 | A/III-B | BRS4 | 36 | CG14484 | 52 | NMBR/GRPR/BR | >700 | ||
| CG14484 | A/III-B | GRPR | 40 | CG14593 | 43 | NMBR/GRPR/BR | >700 | ||
| CG8784 | A/III-B | NMUR | 36 | CG8795 | 117 | NTR/GHSR | >700 | ||
| CG8795 | A/III-B | NMUR | 39 | CG8784 | 114 | NTR/GHSR | >700 | ||
| CG9918 | A/III-B | NMUR | 34 | CG8784 | 61 | NTR/GHSR | >700 | ||
| CG14575 | A/III-B | NMUR | 42 | CG8784 | 39 | C48C5.1 | 39 | NTR/GHSR | >700 |
| CG14003 | A/III? | GHSR | 19 | CG10001 | 20 | NTR/GHSR | <100 | ||
| CG5911A | A/III-B | TRFR | 25 | TRFR | <500 | ||||
| CG5911B | A/III-B | GHSR | 28 | ND | |||||
| CG2114 | A/III? | TRFR | 11 | T14C1.1[iii] | 28 | TRFR | <300 | ||
| CG16726 | A/III? | NK3R | 14 | TRFR | <300 | ||||
| CG6986 | A/III? | TRFR | 10 | CG16726 | 11 | TRFR | <300 | ||
| CG13575 | A/III | GHSR, LSR | 8 | CG10626 | 9 | GPRJ | <300 | ||
| CG8985 | A/III? | AlstR | 4 | CG13803 | 130 | F57B7.1[iii] | 29 | TRFR | <300 |
| CG13229 | A/III? | NY5R | 6 | CG13803 | 46 | F57B7.1[iii] | 31 | TRFR | <300 |
| CG13803 | A/III? | AlstR | 7 | CG8985 | 130 | F57B7.1[iii] | 25 | TRFR | <300 |
| CG5936 | A/III? | NTR | 2 | B0563.6[iii] | 18 | TRFR | <300 | ||
| AlstR | A/V | GALR | 41 | CG10001 | 67 | ZK455.3 | 43 | GALR/GALS/GALT | <500 |
| CG10001 | A/V | GALR | 28 | AlstR | 53 | ZK455.3 | 31 | GALR/GALS/GALT | <500 |
| CG7285 | A/V | SSR2 | 49 | CG13702 | 78 | SSR | <500 | ||
| CG13702 | A/V | SSR2 | 50 | CG7285 | 75 | SSR | <500 | ||
| GRHR | A/V | GRHR | 51 | GRHR/VPR/OXYR | >700 | ||||
| CG10698 | A/V | GRHR | 36 | GRHR | 37 | GRHR/VPR/OXYR | >700 | ||
| CG6111 | A/V | V1BR | 38 | GRHR/VPR/OXYR | >700 | ||||
| Fsh | A/V (c) | LSHR | 66 | FSHR/TSHR/LSHR/LGR4–6 | >990 | ||||
| rk | A/V (c) | LSHR | 68 | FSHR/TSHR/LSHR/LGR4–6 | >990 | ||||
| CG4187 | A/V (c) | GRL101λ | 57 | CG5042 | 43 | LGR7 | >900 | ||
| CG5042 | A/V (c) | GRL101λ | 34 | CG4187 | 40 | LGR7 | >900 | ||
| CG4395 | B/I | CALR | 22 | CG17415 | 24 | CALR/CGRR | >500 | ||
| CG17415 | B/I | CALR | 44 | CALR/CGRR | >500 | ||||
| CG13758 | B/I–III | CALR | 38 | C13B9.4 | 50 | B/I–III | <500 | ||
| CG8422 | B/I | DIHR | 54 | CG12370 | 65 | DIHR | >900 | ||
| CG12370 | B/I | DIHR | 71 | CG8422 | 81 | DIHR | >900 | ||
[i] F/G, Family/Group classification. Diagnostic, Highest scoring receptor with known ligand/function. Fly, Highest scoringDrosophila GPCR (with P value near or exceeding the best diagnostic score). Worm, Highest scoring Caenorhabditis elegans GPCR (with P value near or exceeding the best diagnostic score). P, P value for respective diagnostic, fly or worm GPCR (le−x, where x is the value displayed in the table).
[ii] P value for stable fly TKR is 74.
[iii] Several other C. elegans GPCRs displayed Pvalues exceeding the value obtained for the top “diagnostic” hit. CG10001 tends to be promiscuous on BLASTP with other, even very distantly related, fly receptors. λ, GRL101, as well as the other members of this class of GPCRs, is an orphan receptor (LGR7 is not in the public sequence databases).
[iv] ND, not done. (The remaining receptor abbreviations are described in the legends of Figs. 1 F2 F3.)