Table 2.

Sequences and Characteristics of ET Primers

Marker nameChromosome locationRepeatPCR product size range (bps)Forward primer sequenceReverse primer sequence
IFN-A9p23-9p22di (gt)130–155TGCGCGTTAAGTTAATTGGTTM′GTAAGGT*GGAAACCCCCACT
D9S1719p21-pp21di (ca)150–185AGCTAAGTGAACCTCATCTCTGTCTM′ACCCT*AGCACTGATGGTATAGTCT
D9S1629p22-9p21di (ca)160–205M′GCAAT*GACCAGTTAAGGTTCAATTCCCACAACAAATCTCC
D9S7479pter-9ptertetra (gata)170–210GCCATTATTGACTCTGGAAAAGACM′CAGGCT*CTCAAAATATGAACAAAAT

[i] (M′) Indicates the location of conjugation to the common cyanine donor dye (CYA).

[ii] (T*) Indicates the modified T nucleotide where the acceptor dyes, ROX or R110, were conjugated. (Hung et al. 1996; Hung et al. 1998; Ju et al. 1995)