Table 2.

Identification of New T. cruzi Genes

dbGSS[ii] Description[iii] Score Expect
11462ref-NP_011059.1-GLC7-protein phosphatase type I [Saccharomyces cereviseae]4740.00E+00
10993sp-P22679-elongation factor TU (EF-TU) [Mycoplasma hominis]3120.00E+00
00184sp-P46794-cystathionine beta-synthase [Dictyostelium discoldeum] 3030.00E+00
11438sp-O76767-lumen protein retaining receptor [Drosophila melanogaster]2080.00E+00
11472emb-CAB56598.1-alpha dynein heavy chain [Chlamydomonas reinhardtii]2020.00E+00
11026gi-2425121-Spalten [Dictyostelium discoldeum]1160.00E+00
12036gb-AAF19802.1-N-myristoyl transferase [Brassica oleracea]1070.00E+00
01761gl-3004644-trypanothione synthetase [Crithidia fasciculata]4285.00E-42
11137gb-AAB67249.1-T-complex protein 1, Beta subunit [Homo sapiens]4249.00E-42
11193ref-NP_014850.1-RET1-second-largest subunit of RNA polymerase III [Saccharomyces cereviseae]4221.00E-41
11590gb-AAF08387.1-26S proteasome regulatory complex subunit p48A [Drosophila melanogaster]4192.00E-41
11120emb-CAA65384-malate dehydrogenase [Mesembryanthemum crystallinum]3712.00E-35
11285gi-1931649-DNA helicaso isolog [Arabidopsis thaliana]3532.00E-33
11563ref-NP_013458.1-transaldolase [Saccharomyces cerevisiae]3487.00E-33
09938gi-2246458-S-adenosyl-methionine-sterol-C-methyltransferase [Ricinus communis]3488.00E-33
0705pir-T1017324-sterol C-methyltransferase-castor bean [Ricinus communis]3489.00E-33
11338gb-AAC02737.1-3-hydroxyisobutyryl-coenzyme A hydrolase [Arabidopsis thaliana]3415.00E-32
11467gb-AAF04493.1-acetyl-CoA carboxylase 1 [Toxoplasma gondii] 3307.00E-31
10956dbj-BAA84364.1-DEIH-box RNA/DNA helicase [Arabidopsis thaliana]3282.00E-30
11575gb-AAC73040.1-putative AAA-type ATPase [Arabidopsis thaliana]3235.00E-30
11516sp-P05439-ATP synthase alpha chain [Rhodobacter blasticus]3192.00E-29
11606sp-P51044-citrate synthase, mitochondrial precursor [Aspergillus niger]3212.00E-28
11417gb-AAF21464.1-proline oxidase 2 [Homo sapiens]3102.00E-28
11502gi-2654103-MAPKK kinase [Neurospora crassa]3049.00E-28
11523gb-AAD26855.1-phenylalanyl tRNA synthetase beta subunit [Mus musculus]3012.00E-27
11568gi-4101722-histone deacetylase mHDA1 [Mus musculus]3012.00E-27
11480gi-2462752-phosphatidylinositol 3-kinase [Arabidopsis thaliana]2994.00E-27
11463sp-P32826-serine carboxypeptidase precursor [Arabidopsis thaliana]2994.00E-27
10965gi-687208-dynein heavy chain isotype 5C [Tripneustes gratilla]2895.00E-26
11328pir-A56220-protein kinase aurora-fruit fly [Drosophila melanogaster]2871.00E-25
11446sp-O15228-dihydroxyacetone phosphate acyltransferase (DAP-AT) [Homo sapiens]2807.00E-25
11433gb-AAF11511.1-acetyl-CoA acetyltransferase [Deinococcus radiodurans]2799.00E-25
11601sp-P30575-enolase 1 (2-phosphoglycerate dehydratase) [Candida albicans]2781.00E-24
01810sp-O94476-eukaryotic translation initiation factor 6 (EIF-6) [Schizosaccharomyces pombe]2753.00E-24
0740gb-AAF62506.1-ribosomal protein LS [Trypanoplasma borreli]2735.00E-24
11274pir-S70896-aminomethyltransferase [Saccharomyces cerevisiae]2715.00E-24
11279gi-780410-helicase [African swine fever virus]2726.00E-24
11882sp-Q07405-ATP synthase alpha chain [Myxococcus xanthus]2691.00E-23
11432emb-CAB40791.1-centrin [Euplotes octocarinatus]2653.00E-23
11654gi-1872473-delta-24-sterol methyltransferase [Triticum aestivum]2587.00E-23
01015pir-A56492-protein kinase ERK2 [Dictyostelium discoideum]2808.00E-23
11584ref-NP_005678.1-phenylalanyl-tRNA synthetase beta-subunit [Homo sapiens]2591.00E-22
11434sp-O05593-methionyl-tma synthetase [Mycobacterium tuberculosis]2546.00E-22
11038gi-1354084-axonemal dynein light chain p33 (Strongylocentrotus purpuratus]2512.00E-21
11440gi-2665637-mismatch repair protein MSH6 [Mus musculus]2484.00E-21
11248gb-AAF22155.1-ARD-1 N-acetyltransferase homologue [Mus musculus]2441.00E-20
11852gb-AAC32590.1-sperm flagellar protein Repro-SA-1 [Homo sapiens]2395.00E-20
01825dbj-BAA20996-kinesin-like protein [Caenorhabditis elegans]2396.00E-20
11210pir-A35630-regulatory protein algR3 [Pseudomonas aeruginosa]2377.00E-20

[i] GSS sequences were used to search NCBI's non-redundant database using BLASTX. The first 50 GSSs out of the 947 GSSs showing matches against non-T. cruzi sequences are listed. Detailed information about the homologies found for GSSs can be found athttp://www.iib.unsam.edu.ar/genomelab/tcruzi/gss.html.

[ii] GSS names in dbGSS are the numbers given here preceded by GSSTc (e.g., GSSTc11210).

[iii] Descriptions are taken directly from the BLAST reports.