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Table 1.

Microdevice Sequencing Error Rate as a Function of Base Number

Sample	35– 100	101– 150	151– 200	201– 250	251– 300	301– 350	351– 400	401– 450	451– 500	501– 550	551– 600	Read length	Run time (min)
1	3	0	0	0	0	0	0	0	4	11	25	415	22.9
2	0	0	0	0	0	0	0	0	0	4	21	515	22.7
3	1	0	0	0	0	0	0	0	0	2	17	520	22.8
4	0	0	0	0	0	0	0	0	0	5	19	515	21.1
5	0	0	0	0	0	0	0	4	10	27	—	425	20.5
6	1	0	1	0	0	3	0	1	4	8	21	365	20.5
									Average (RSD)		460 (66}	21.7 (1.2)
7	0	0	0	0	0	0	0	0	0	0	1	565	25.7
8	3	0	0	0	0	0	0	0	1	5	7	465	27.3
9	2	0	0	0	0	0	0	0	1	4	7	515	28.8
10	4	0	0	0	0	0	0	0	0	2	7	500	26.1
11	3	0	0	0	0	0	0	0	2	5	10	470	26.1
12	3	0	0	0	0	0	0	0	1	1	4	515	26.6
									Average (RSD)		505 (36}	26.3 (1.2)

[i] Error rate after base-calling by Trout, manual editing, and comparison with ABI 377 data. Read length was defined as the length of sequence in bases that had a base-calling accuracy of at least 99%. Samples 1–6 were run at 200 V/ cm with 4% LPA (C1); samples 7–12 were run at 150 V/cm with 3% LPA (C2) at 45°C on a microdevice of 11.5 cm effective separation length. Samples were from the WICGR production line using DNA from chromosome 17.