Table 1.

α-Galactosidase Actvities in Cell Lysates of Different Strains Grown on C + Y Medium Supplemented with the Indicated Carbon Source

Strain[i] Carbon source α-Galactosidase activity (U/mg protein)[ii]
sp. R6x (wild type)glucose0.7 ± 0.1
glucose/sucrose0.4 ± 0.1
glucose/raffinose200 ± 40
sucrose/raffinose0.7 ± 0.2
sp. R6x (ΔptsII)glucose0.8 ± 0.1
glucose/raffinose220 ± 38
sucrose/raffinose203 ± 45
sp. R6x (ΔccpA)glucose0.7 ± 0.1
glucose/raffinose238 ± 44
sucrose/raffinose1.0 ± 0.4
sp. R6x (ΔrafR)glucose0.4 ± 0.1
glucose/raffinose34 ± 8
sp. R6x (ΔrafS)glucose0.8 ± 0.1
glucose/raffinose444 ± 92
E. coli L broth0
E. coli pR326agaL broth800 ± 120
E. coli rafR/agaL broth4600 ± 410

[i] (sp.) S. pneumoniae.

[ii] The activities are expressed in units (nmoles ofp-nitrophenol produced per min of incubation at room temperature) per mg protein with their s.d. Shown is the mean of three experiments.