Base-Call Accuracy as a Function of Target Complexity, Probe Length, and Ligation Method
| Gene or fragment | ||||||||
| 8-mer | 535-bp cftr | 1.2-kb HIV pol | 2.5-kb cytochrome b | 5.4-kb φX174 | ||||
| 9-mer | ||||||||
| Enzymatic | 99.0 (0.2) | 98.2 (0.1) | 88.9 (1.3) | 73.9 (4.1) | ||||
| 99.8 (0.0) | 99.9 (0.0) | 94.5 (0.3) | 85.2 (0.5) | |||||
| Chemical | 99.0 (0.4) | 96.1 (0.5) | 87.8 (1.5) | 69.0 (2.8) | ||||
| 100.0 (0.0) | *99.5 (0.0) | 93.4 (0.2) | 89.0 (0.4) | |||||
[i] Percentage of correct calls followed by the percentage of incorrect calls in parentheses.
[ii] Enzymatic ligation employed standard T4 DNA ligase conditions. A confidence threshold of 1.15 was used for the base-calling. The analysis procedures are described in Methods. No calls + incorrect calls + correct calls = 100%. (*) s.d. = 0.1% (n = 4 experiments).