Summary of Most Abundant ESTs by Library as Determined by Cluster Analysis
| Rank | RH tachyzoite cDNA library | ME49 tachyzoite cDNA library | ||||
| dbEST ID no. | no. | gene/description | dbEST ID no. | no. | gene/description | |
| 1 | 465887 | 107 | GRA1 p24 dense granule protein | 571382 | 42 | SAG1 p30 surface antigen |
| 2 | 466589 | 69 | GRA2 p28 dense granule protein | 571076 | 32 | GRA2 p28 dense granule protein |
| 3 | 465733 | 68 | GRA6 p32 dense granule protein | 571569 | 29 | GRA1 p24 dense granule protein |
| 4 | 465767 | 64 | SAG1 p30 surface antigen | 571208 | 28 | unknown (similar to RH 467174) |
| 5 | 465877 | 58 | GRA5 p21 dense granule protein | 571108 | 27 | SAG2 p22 surface protein |
| 6 | 466942 | 53 | SAG2 p22 surface antigen | 571574 | 17 | GRA5 p21 dense granule protein |
| 7 | 467174 | 34 | unknown (similar to ME49 571208) | 571542 | 15 | unknown (similar to RH 467213 |
| 8 | 465857 | 29 | S8 ribosomal protein | 571282 | 15[iii] | unknown (similar to RH 466613) |
| 9 | 466627 | 28 | GRA3 p30 dense granule antigen | 571237 | 11[iii] | unknown (similar to RH 466242) |
| 10 | 466406 | 26[ii] | unknown (similar to ME49 574388) | 571154 | 9 | GRA6 p32 dense granule protein |
| 11 | 465750 | 22 | unknown (similar to ME49 571377) | 571377 | 9 | unknown (similar to RH 465750) |
| 12 | 466392 | 21 | 35-kD toxoplasma polypeptide | 571392 | 9 | unknown (similar to Schizosaccharomyces pombe7.67961) |
| 13 | 467170 | 19 | unknown (similar to ME49 604952) | 571332 | 8 | S2 ribosomal protein |
| 14 | 465671 | 18 | S30 ribosomal protein | 571072 | 8 | L18a ribosomal protein |
| 15 | 466619 | 17 | S7–S8 ribosomal protein | 574528 | 8 | L22 ribosomal protein |
[i] Total number of ESTs for the RH tachyzoite cDNA library was 5359; total number of ESTs for the ME49 tachyzoite cDNA library was 1806.
[ii] EST frequency is fivefold more abundant in RH.
[iii] EST frequency is fivefold more abundant in ME49.