Characterization and Mutation Analysis of Transcripts Located between PYGM and D11S4933
| Transcripts | GenBank ID no. | Size of mRNA (kb) | cDNA cloning | Mutation analysis of FMEN 1 patients a | Uni- Gene b | Gene Map c | |||||||
| Southern | ddF | ||||||||||||
| Nu | EST06996 | 4.5 | incomplete | not done | not done | yes | no | ||||||
| GC-kinase | U07349 | 2.9 | GenBank | yes | yes | yes | no | ||||||
| Mu | U93236 | 2.8 | complete | yes | yes+ | yes | yes | ||||||
| Kappa | AF001543, AF001544 | 8 | incomplete | not done | partial+ + | no | no | ||||||
| HPAST | AF001434 | 3.5 | complete | yes | yes | yes | yes | ||||||
| Xi | AF001545 | 8 | incomplete | not done | not done | no | no | ||||||
| PP2AB56β | L42374 | 2.5 | GenBank | yes | yes | yes | yes | ||||||
| ARL2 | L13687 | 0.9 | GenBank | yes | yes | yes | no | ||||||
| Iota | AF001435 | 2.2 | complete | not done | not done | yes | yes | ||||||
| Gamma | AF001547 | no signal | 1.6 kb | not done | not done | yes | yes | ||||||
| Lambda | AF001891 | 1.4/3.5* | incomplete | not done | not done | yes | no | ||||||
| NOF1 | U39400 | 2.2 | GenBank | yes | yes | yes | yes | ||||||
| DNA polα | L24559 | 2.2 | GenBank | yes | yes | yes | yes | ||||||
| Zeta | AF001436 | 2.0/2.2* | complete | yes | yes | yes | yes | ||||||
| HREQ | AF001433 | 2.5 | complete | yes | not done | yes | yes | ||||||
| Eta | AF001546 | no signal | 1.2 kb | not done | partial+ + + | yes | yes | ||||||
| Theta | N55476,R19205 | 4 | incomplete | not done | not done | yes | no | ||||||
| Epsilon/Beta | AF001892,AF001893 | 4.4/19.0* | incomplete | yes | not done | yes | yes | ||||||
| Alpha | AF001540, AF001541, AF001542 | 8.5 | incomplete | not done | not done | yes | yes | ||||||
| Delta | W37647 | 0.7/2.8* | incomplete | not done | not done | yes | no | ||||||
| MLK3 | L32976 | 3.5 | GenBank | yes | yes | yes | yes | ||||||
| RELA | L19067 | 2.5 | GenBank | yes | yes | yes | yes | ||||||
| TIP60 | U40989 | 2.9 | GenBank | yes | not done | yes | yes | ||||||
[i] Four known genes from this region, ZFM1 (D26120),FAU1 (X65923), CAPN1 (X04366), and OVO(A. Chidambaram, R. Allikmets, S.C. Chandrasekharappa, S.C. Guru, W. Modi, B. Gerrard, and M. Dean, in prep.), were not included here as they have previously been excluded as candidates for MEN 1. (*) Two messages on Northern blot.
[ii] (+)Mutation analysis by dideoxy fingerprinting identified Mu as the MEN1 gene (Chandrasekharappa et al. 1997); (++) 22 exons excluded (+++) available 1.2 kb, excluded.
[iii] Presence of EST matches with the transcript in Unigene is indicated by ‘yes’ and absence by ‘no’ (http://www.ncbi.nlm.nih.gov/Schuler/UniGene/Chr11.html).
[iv] Presence of EST matches with the transcript on the Gene Map is indicated by ‘yes’ and absence by ‘no’ (http://www.ncbi.nlm.nih.gov/SCIENCE96/ResTools.html).