YAC Contigs Constituting the Human Chromosome 7 Physical Map
| Anchored YAC contigs[i] | ||||||||
| Contig designation[ii] | Contig name[iii] | Genetic interval[iv] | RH interval[v] | Oriented[vi] | No. STSs[vii] | No. YACs[viii] | Cytogenetic[ix] | Physical length[x] |
| A | sWSS1361 | yes | 5 | 17 | 7p22 | 1,450 | ||
| B | sWSS457 | 3 | no | 6 | 11 | 550 | ||
| C | sWSS173 | 5–14 | 1.01–8.01 | yes | 86 | 148 | 7p22 | 6,150 |
| D | sWSS244 | 16–18 | 9.01–9.02 | yes | 20 | 25 | 7p21–p22 | 1,450 |
| E | sWSS9 | 19–87 | 9.03–59.02 | yes | 634 | 1260 | 7p11.2–p21 | 49,600 |
| F | sWSS3 | 88–97 | 60.01–65.01 | yes | 145 | 426 | 7p11.2–q11.2 | 10,100 |
| G | sWSS726 | 97 | 66.04–66.05 | yes | 6 | 8 | 1,300 | |
| H | sWSS686 | 97–98 | 65.02–65.03 | yes | 13 | 19 | 7q11.2 | 1,150 |
| I | sWSS86 | 100 | 66.01–66.06 | yes | 55 | 114 | 7q11.2 | 1,950 |
| J | sWSS10 | 101–128 | 67.01–77.07 | yes | 334 | 582 | 7q11.2–q22 | 27,550 |
| K | sWSS381 | 128–129 | 77.01–77.03 | yes | 30 | 41 | 7q21–q22 | 3,100 |
| L | sWSS11 | 129 | 78.04–78.06 | yes | 6 | 8 | 7q22 | 1,300 |
| M | sWSS328 | 129 | 78.05–78.07 | yes | 7 | 6 | 1,900 | |
| N | sWSS6 | 130–140 | 78.01–80.05 | yes | 111 | 246 | 7q22 | 10,550 |
| O | sWSS26 | 141–145 | 81.01–86.01 | yes | 95 | 225 | 7q22–q32 | 10,300 |
| P | sWSS1095 | 146 | 86.02 | no | 2 | 5 | 1,250 | |
| Q | sWSS81 | 145–150 | 87.01–96.03 | yes | 93 | 198 | 7q31–q32 | 6,400 |
| R | sWSS8 | 149–185 | 93.01–101.02 | yes | 359 | 704 | 7q31–q35 | 30,350 |
| S | sWSS806 | 99.04 | no | 6 | 5 | 800 | ||
| T | sWSS31 | 186–196 | 103.01–104.01 | yes | 71 | 137 | 7q36 | 4,450 |
| U | sWSS189 | 201–204 | 105.01–107.04 | yes | 25 | 39 | 7q36 | 2,450 |
| V | sWSS25 | yes | 8 | 19 | 7q36 | 1,150 | ||
| Unanchored YAC contigs[xi] | ||||||
| Contig name[iii] | No. STSs[vii] | No. YACs[viii] | Cytogenetic[ix] | Physical length[x] | Genes/ESTs[xii] | |
| sWSS33 | 3 | 2 | 7q36 | 1,550 | EST (1) | |
| sWSS419 | 3 | 5 | 7 CEN | 900 | ||
| sWSS420 | 2 | 3 | 7 CEN | 700 | ||
| sWSS1035 | 7 | 11 | 1,950 | gene (1) | ||
| sWSS1240 | 3 | 1 | 7q36 | 550 | ||
| sWSS1463 | 2 | 1 | 7q22 | 250 | gene (1), EST (1) | |
| sWSS2100 | 1 | 1 | 250 | |||
| sWSS2444 | 3 | 1 | 250 | |||
| sWSS2808 | 1 | 2 | 1,400 | |||
| sWSS3161 | 2 | 2 | 250 | EST (2) | ||
| sWSS3309 | 1 | 1 | 300 | EST (1) | ||
| sWSS3384 | 3 | 7 | 7q11.2 | 1,000 | EST (3) | |
| sWSS3577 | 1 | 1 | 350 | EST (1) | ||
| sWSS3647 | 1 | 2 | 1,500 | EST (1) | ||
[i] Contigs anchored in place relative to one another, based on their position with respect to the Genethon genetic, chromosome 7 RH, and/or cytogenetic maps (see text for details). Note that only the first and last contigs [A (sWSS1361) and V (sWSS25), respectively] are anchored exclusively on the cytogenetic map; these two contigs contain YACs corresponding to the 7p and 7q telomere, respectively.
[ii] Designation for the contig based on its relative position on the physical map (ordered from A to V, starting at 7pter and ending at 7qter).
[iii] Contig name assigned by SEGMAP, corresponding to the lowest numbered STS in the contig.
[iv] Interval on the Genethon genetic map spanned by the contig, corresponding to the position(s) [in cumulative cM from 7pter to 7qter (see Fig. 2)] of the most proximal and distal Genethon genetic marker(s) contained in the contig.
[v] Interval on the chromosome 7 RH map spanned by the contig, corresponding to the position(s) [in ordered bin location from 7pter to 7qter (see Fig. 3)] of the most proximal and distal RH-mapped marker(s) contained in the contig.
[vi] A contig is considered oriented relative to the centromere and telomeres if (1) the positions of uniquely ordered Genethon genetic markers contained in the contig allow the proximal and distal ends to be defined; (2) the positions of uniquely ordered RH-mapped markers contained in the contig allow the proximal and distal ends to be defined; or (3) in the case of contigs A (sWSS1361) and V (sWSS25), the position of the telomere-containing YAC indicates the telomeric end. The rare discrepancies between the physical map and the Genethon genetic or RH maps (see Figs. 2 and 3) were not considered in assessing contig orientation. In virtually every instance, there is remarkable agreement between the orientation indicated by the Genethon genetic and RH maps. For three contigs (B, P, S), none of the three criteria listed above were met, and the contigs are thus not considered oriented. Similarly, none of the unanchored contigs are considered oriented.
[vii] Number of STSs contained in the contig.
[viii] Number of YACs contained in the contig. Note that the sum of the YACs contained in each contig exceeds the total number of YACs actually present in the physical map (Table 4); this is because of the presence of YACs mapping to more than one contig. Here, such YACs are counted each time they appear in a contig, whereas in Table 4, each YAC is counted only once.
[ix] Established cytogenetic position of the contig, based on FISH-based mapping of a YAC(s) from the contig (Green et al. 1994,1995).
[x] Estimated physical length of the contig (in kb), as calculated by SEGMAP in deducing a computed map (see text) and then rounded to the nearest 50 kb.
[xi] Contigs unanchored in place relative to each other or to the anchored contigs. These contigs are devoid of a Genethon genetic or RH-mapped marker. Although many have cytogenetic assignments, there is not sufficient information to deduce a more refined position.
[xii] Presence of gene- and/or EST-specific STSs within the contig is indicated, along with the number of such STSs (in parenthesis).