Cosmids for Which Transgenic Strains Could Not Be Constructed Using Standard Injection Conditions
| Cosmid | [Cosmid DNA] used to construct transgenic strain[ii] (ng/μl) |
| C08C3 | 10 |
| F28F5 | 10 |
| F37C12 | 7 |
| F57B9 | 5 |
| R01H2 | 7 |
| ZK512 | 10 |
[i] Our standard injection conditions are defined as 20 ng/μl of cosmid DNA and 80 ng/μl of pCes1943. Listed are the lower concentrations of cosmid DNA that were used to generate transgenic strains successfully.
[ii] Cosmid concentrations are estimates only, based on either gel quantification or information provided by the Sequencing Consortium (see Methods). Final concentrations of injection DNA were made up to 100 ng/μl with pCes1943.