The overall sequence throughput and aligned coverage is shown for each sequencing technology used in the study. We also report the number of spurious and missed mutations observed from each experiment.

^aFor the 454 and Illumina technologies, the total number of reads reflects the number of reads that remained after manufacturer quality controls. The Applied Bisoystems (AB) read totals reflect all reads produced by the sequencing run.

^bThe coverage produced by those reads in the second column that passed the mapping filters we used for each technology (Methods).

^cEstimated number of reads based on in silico subsampling of coverage.