Primer Design
| SNP name | Type | Allele-specific primers | Label[i] | Reverse primers | Melting temperature | dbSNP ID (ss#) |
| 216 S2 | G/C | ACCAGGCCAGGCCGGCC | Tam | TGGCCCTGTGCAGGCTGAAA | 68 | SS5112738 |
| TGCTGCCTCTGCTCCCAGGC | Fam | GGGAACCGCAGGAGTAGGCTCA | ||||
| 216 M+1 | G/T | AAGGTTCAGGGTGAGGGTTTCGT | Tam | NA (Linear Assay) | SS5112737 | |
| AAGGTTCAGGGTGAGGGTTTCGC | Fam | |||||
| 216 ST+4 | T/G | CAGCATTTGGGAACTTCAAGT | Fam | CAAATCACCTCTGTCACCCC | 59 | SS5112741 |
| GTCCACTCTCCTGCTTCTCC | Rox | CCACCCAGGTCACAGAGAAC | ||||
| 216 ST+7 | T/C | TTTGACTGTCCCCATCCCATCT | Fam | GTCTGTGGGCAGAGGAGCAA | 64 | SS5112744 |
| TGACTGTCCCCATCCCATCC | Rox | |||||
| 216 V-2 | A/G | ACTGAGTGAGGATGGGCTCT | Tam | TGCAGTTCAAGTTCCTGGAG | 59 | SS5112757 |
| CTGAGTGAGGATGGGCTCC | Fam | |||||
| 216 S+1 | T/A | GTGGCCTCCCAGTCAAGCGT | Tam | GTCAAGGGTGAGGCAGCCAG | 60 | SS5112740 |
| TGGGGCAGGGATCCACCCCCT | Fam | CGAGCGCCTGCTTCCTGAGCC |
[i] (Tam) TAMRA; (Fam) 6FAM.