Markup | Genome Research

Table 1.

MPERs in Various Genomic Regions

Gene/location		Characteristics of the region analyzed		MPER (number of errors/site/21.6 generations)		Fidelity (%/site/generation)
Gene/location		G + C content (%)	CpG score	MPER (number of errors/site/21.6 generations)		Fidelity (%/site/generation)	Methylation status
E-cadherin
CGI-P	64.6	0.70	U	0.022 ± 0.012	99.89
CGI-outside	66.7	0.66	U	0.053 ± 0.012^***	99.75
Non-CGI	50.2	0.59	M	0.004 ± 0.005^*	99.98
p41-Arc
CGI-P	68.9	0.69	U	0.032 ± 0.010	99.85
CGI-outside	66.4	0.72	U	0.091 ± 0.039^**	99.56
Non-CGI	65.2	0.31	M	0.017 ± 0.012^*	99.92
SIM2
CGI-P	65.7	0.76	U	0.018 ± 0.004	99.92
CGI-outside	64.8	0.70	U	0.037 ± 0.008^***	99.83
3-OST-2
CGI-P	66.1	0.80	U	0.021 ± 0.008	99.90
Cyclophilin A
CGI-P	61.6	0.90	U	0.032 ± 0.017	99.85
MAGE-A3
CGI-P	63.7	0.46	M	0.002 ± 0.004	99.99
H19-DMR
both alleles	59.5	0.65	U/M	0.026 ± 0.016	99.87
unmethylated			U	0.043 ± 0.034	99.80
methylated			M	0.007 ± 0.011^*	99.98

[i] MPERs (number of errors/site/21.6 generations) were calculated from the observed number of errors in six clonal populations that underwent 21.6 generations. Fidelity (%/site/generation) was calculated by the equation M = 1 −F ^21.6. MPERs in CGIs outside the promoter regions and non-CGI regions were compared with those in the promoter regions by the t-test, *P < 0.05, **P < 0.01, ***P < 0.005. CGI-P: CGI in the promoter region; CGI-outside: CGI outside the promoter regions; Non-CGI: CpG sites outside CGIs. G + C content and CpG score of the regions analyzed were calculated for the most suitable region larger than 500 bp using a program at the “CpG Island Searcher” web site (http://www.uscnorris.com/cpgislands/). Methylation statuses of these regions in physiological conditions were described as U: unmethylated, M: methylated.