[i] Each screening plate represents ∼750 uniqueM ₂ individuals pooled eightfold in a 96-well plate. For each pool plate, the number of ∼1-kb gene fragments screened is listed. For each gene order, at least four pool plates were screened. The number of total mutations identified and the average number of mutations identified on a particular plate are listed (not every mutation identified in this table has been sequenced). The total screened fragment length represents the sum of gene fragment lengths screened minus ∼140 bp per target (compensating for the regions near primers where mutation detection is less efficient). The total bases screened is calculated by the total screened fragment length times the number of plants screened (750). This number represents the total number of bases interrogated per screening plate. The number of predicted mutations per M ₂ plant is calculated as the total genome size (125 Mb)/(total bases screened/total mutations). This calculation assumes an even distribution of EMS mutagenesis in both genic and intergenic regions and does not compensate for differences in GC content throughout the genome. The mean embryo lethal C class score is a measure of the percent “normal” siliques in theM ₁ generation of each population. Embryo lethal C class scores are not available (NA) for pool plates 91–127 as individuals represented on these plates derive from multiple different mutagenesis trials.