PCR Primers, Product Size (bp), and Array-Bound Allele-Specific Oligonucleotide Probes Used for Genotyping
| Locus | Function | Sequence | Size (bp) | GC% | T |
| −122 | F-PCR | 5′-Cy5-GGGGTCACCTGACCTTTCTC-3′ | 178 | ||
| R-PCR | 5′-GACTGACGGGCAAAGTTCTC-3′ | ||||
| ASO-122-N | 5′-CGTCCTCTGA A CACCCCAGCT-3′ | 62 | 62 | ||
| ASO-122-M | 5′-CGTCCTCTGA G CACCCCAGCT-3′ | 67 | 65 | ||
| R353Q | F-PCR | 5′-TCTGTGCCGGCTACTCG-3′ | 150 | ||
| R-PCR | 5′-Cy5-TGTACACCCCAAAGTGGCC-3′ | ||||
| ASO R353Q-N | 5′-CCACTACC G GGGCACGT-3′ | 71 | 60 | ||
| ASO R353Q-M | 5′-CCACTACC A GGGCACGT-3′ | 65 | 57 | ||
| C282Y | F-PCR | 5′-TGGCAAGGGTAAACAGATCC-3′ | 390 | ||
| R-PCR | 5′-Cy5-CTCAGGCACTCCTCTCAACC-3′ | ||||
| ASO 282-N | 5′-AGATATACGT G CCAGGTGGAG-3′ | 52 | 57 | ||
| ASO 282-M | 5′-AGATATACGT A CCAGGTGGAG-3′ | 47 | 54 | ||
| H63D | F-PCR | 5′-ACATGGTTAAGGCCTGTTGC-3′ | 208 | ||
| R-PCR | 5′-Cy5-GCCACATCTGGCTTGAAATT-3′ | ||||
| ASO-63-N | 5′-GTTCTATGAT C ATGAGAGTCG-3′ | 43 | 51 | ||
| ASO-63-M | 5′-GTTCTATGAT G ATGAGAGTCG-3′ | 43 | 51 |
[i] F, forward; R, reverse; N and M, wild-type and mutant allele-specific oligonucleotides, respectively. Bolded underlined base indicates site under interrogation.
[ii] Calculated using Primer Express v1.5 (Applied Biosystem Group).