Table 1.

Constructions and Plasmids

Plasmids Description Reference or source (GenBank accession number)
pNOT218pTZ18R containing two in-frameNotI sites boarding the MCS  polylinker, ApR Rowe-Magnus et al. 2001 (AF480833)
pTZ19RColE1 replicon ApRPharmacia (Y14835)
pSU19p15A replicon CmRBartolome et al. 1991 (X53939)
pUC18ColE1 replicon ApRNorrander et al. 1983 (L08752)
pCR2.1ColE1 replicon ApR KmRInvitrogen
Vibrio metschnikoviiinserts
p253pUC18 BamHI (genomic BglII 4.0-kb fragment)This work (AF525313)
p273pUC18EcoRI (genomic EcoRI 4.6-kb fragment)This work (AF530480)
P372pUC18 BamHI (genomicBglII 4.3-kb fragment)This work (AY141193)
P374pNOT218 (genomicEcoRV–BspEI 6.4-kb fragment)This work (AY014398)
V. fischeriinserts
p641[i] pUC18 BamHI (genomicBglII 2.1-kb fragment)This work (AF177199)
p667[i] pUC18 HindIII (genomicHindIII 3.3-kb fragment)This work (AF177199)
p668pUC18 HindIII (genomicHindIII 3.9-kb fragment)This work (AY178758)
p669pUC18 XbaI (genomic XbaI 4.5-kb fragment)This work (AY181031)
p672pUC18XbaI (genomic XbaI 3.5-kb fragment)This work (AY181032)
p789pMTL22 NsiI (genomicNsiI 6.0-kb fragment) Rowe-Magnus et al. 2001(AY014400)
p1357pNOT218 PstI (p669NsiI 739-bp fragment carryingccdAB Vfi  in anti-lacZorientation)This work
p1400pTZ19REcoRI-HindIII (p1357EcoRI-HindIII insert; ccdAB Vfiin  lacZ orientation)This work
p1446pSU19 AccI–EcoRI (p1400AclI–EcoRI 590-bp fragment,  carryingccdB Vfi * R35opal)This work (AY181033)
V. natriegensinsert
p1216[ii] pCR2-1 ([LPR1-2 + INT8-2] PCR product 1.8 kb)This work (AY181034)
p2018[ii] pCR2-1 ([Vna1 + ORF474] PCR product 1.25 kb)This work (AY181034)
V. vulnificus insert
p1184[iii] pCR2-1 ([LPR1 + I456/5] PCR product 2.7 kb)This work (AF539751)
p2029[iii] pCR2-1 ([RPLT + Vvu1] PCR product 1.4 kb)This work (AF539751)
Listonella anguillarum insert
p1456[iv] pUC18HindIII (genomic HindIII 2.7-kb fragment)This work (AY126447)
p2010[iv] pCR2-1 ([RPLT + Lang1] PCR product 0.63 kb)This work (AY126447)

[i] p641 and p667 inserts have a 1354-nt overlap and have been deposited as a single file in GenBank.

[ii] p1216 and p2018 inserts overlap and have been deposited as a single file in GenBank.

[iii] p1184 and p2029 inserts overlap and have been deposited as a single file in GenBank.

[iv] p1456 and p2010 inserts overlap and have been deposited as a single file in GenBank.