Table 1.

Summary of Sequenced γ1230Library Clones

Region Clones Sequences
no. avg. size (kb) total (kb)[i] contig size (bp)[ii] type sequence (bp)[iii]
Beagle(Motu 1)162.852.58043L AATAT726
Beagle 7063
R AATAT254
F (Motu 2)173.371.46431L AATAT942
F 4713
R AATAT776
412 (Motu 3)322.782.49149L AATAT970
412 7572
R AATAT607
Bel (Motu 4)132.646.56888L AATAT582
Bel 6129
R AATAT177
You (Motu 5)51.724.77568L AATAT679
5333
R AATAT [iv]950
R TTCTC [iv]606
Maupiti 1012.9210.239,125L TTCTC201
Maupiti 38,924
Pure AATAT21.53.7 [v]1058AATAT [v]1058
Pure TTCTC40.44.2 [vi]1627TTCTC [vi]1627
CEN Subtotals1902.8495.679,889Complex69,734
Satellite10,155
Breakpoint[vii] 202.0272655Euchromatin2291
R AATAT364
Subtelomeric and Euchromatic352.447.3 [viii]NA [viii]NA [viii]NA
Unassigned2143.0378.0 [viii]NA [viii]NA [viii]NA
γ1230Totals4592.6947.982,544Complex72,025
Satellite10,519

[i] Total amount of sequence generated from all clones for each region.

[ii] Size of contig assembled for each region. L and R indicate position of the satellite to the left and right of the complex DNA (predominantly transposons), respectively (Fig. 2); see Figure 2 for transposon orientations.

[iii] Amount of satellite DNA sequence in the contig corresponding to the indicated junction.

[iv] At the 3′ end of the You contig, the transposon is juxtaposed to a 950-bp block of AATAT satellite, which in turn is juxtaposed with a block of TTCTC satellite.

[v] Total for partial sequences from two clones, which both contained contigs of 529 bp.

[vi] Total for complete sequences from four clones, which contained contigs of 347, 439, 500, and 500 bp.

[vii] The breakpoint between the centromere and euchromatin produced during the generation of γ1230, see Figure 1. The AATAT satellite present in this clone marks the 5′ end of the centromere, but is not included in the CEN TOTALS for simplicity.

[viii] Assemblies of various sizes were produced, but are not included here because they are not relevant to centromere structure.