Table 1.

Products from cleavage of IL 12 polymerase chain reaction product with uracil-N-glycosylase.

Expected fragments[i] Expected mass (da) Allele in which fragment is seen Observed mass (Da)
+/+ +/− −/−
GCC1005.6+/−1007.381004.931006.13
CAA1013.6N/O1012.411014.22
CGA1029.6+1028.691028.57N/O
AAA1037.6+/−1035.851036.691037.89
AGA1053.6N/O1053.691055.88
GGG1085.6+/−1085.971084.611088.01
AGAC1342.8+/−1343.551340.091343.63
AGGA1382.8+/−1379.171382.131383.46
CACAA1616.0+/−1615.271615.741617.10
CAAGC1632.0+1629.591632.72N/O
AAACA1640.0+/−1636.751639.141640.87
AAAGA1680.0+/−1679.711678.971680.74
AAAACG1969.2+/−1965.751969.361971.46
GAAACAA2282.4+/−2284.102285.292282.65
AAACGAAA2595.6+/−2591.632594.182597.34
GCAAAAAAA2908.8+/−2906.312912.222908.53
AGAAACGACA3214.0+/−3210.343211.753216.05

[i] Fragments >2 bp, excluding primer fragments. N/O = not observed.