Advantages and Disadvantages of Detection Strategies Based upon SYBR Green I, FRET, and iFRET
| SYBR Green I | FRET | iFRET | |
| DASH compatible | Yes | Yes | Yes |
| Cost | Low | High | Medium |
| Fluorescence signal | Medium | Low | High |
| T m peak separation | Medium | High | Medium |
| Multiplex potential | No | Yes | Yes |