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Table 1.

Localization of Functionally Characterized Binding Sites to Conserved Blocks

		Location	Ref	Element sequence	NFAT (%ID)	AP-1 (%ID)
IL-2	E1	280	(15)	`AGGAAAATTTGTTTCATA`	90.48	90.48
	E2	160	(15)	`AGAAATTCCAGAGAGTCA`	90.48	95.24
	E3	135	(15)	`AGGAAAAACAAAGGTAAT`	95.24	80.95
	E4	90	(15)	`TTGAAAATATGTGTAATA`	95.25	100
	E5	45	(15)	`TGGAAAAAT`	95.25	n/a
IL-3	E6	306	(11)	`TGAGCTGAGTCAGGCTTCCCCTT`	76.2[ii]	71.43
IL-4	P0	−106	(1, 2)	`GTAAACTCATTTTCCCTTGGTTTC`	95.25	100
	P1	−121	(1, 2)	`GTAATAAAATTTTCCAATGTAAAC`	95.25	100
	P2	−124	(1, 2)	`ACAGGTAAATTTTCCTGTGAAATC`	95.25	100
	P3	−238	(1, 2, 3)	`GGTGTTTCATTTTCCAATTTGTCT`	95.25	95.24[ii]
	P4	−287	(1, 2)	`TATGGTGTAATTTCCTATGCTTGA`	100	100[ii]
	P5	−406	(16)	`GCAGTCCTCCTGGGGAAAGATAGAGTAATATCA`	95.25	95.24
IL-5	E7	163	(4, 6, 7)	`GCATTGGAAACATTTAGTTTCACGAT`	80.95	80.95
	E8	104	(4, 5)	`GAAATTATTCATTTCCTCAAAG`	90.48	95.24
IL-13	E9	135	(12)	`CTGGATTTTCCA`	85.71	n/a
	E10	154	(13)	`CATGAGAAATCAAATCTTTCCTTTA`	90.48	80.95
GMCSF	E11	−67	(9, 10)	`CACCATTAATCATTTCCTCTG`	80.95	100
	E12	−101	(14)	`AGGAGATTCCACAGTTCAGGTAGTTCCCCCGCCTCC`	95.1	90.48
	E13	−163	(13)	`CCTAGGGAAAAGGCTCACCGT`	80.95	90.48
	E14	−2.8kb	(8)	`GCCCTGATGTCATCTTTCCATGA`	90.48	90.48
	E15	−2.9kb	(8)	`CCATCGGAGCCCCTGAGTCAGCAT`	85.71[ii]	85.71
					GATA-3 (%ID)	Distance (bp)
IL-4	E16	−87	(17)	`ATTACACCAGATTGTCAGTTA-`	95.25	20
				`TTCTGGGCCAATCAGCACC`	100
IL-5	E17	−75	(18)	`CTCTATCTGATTGTTA`	95.24	5
					95.24

[i] This table represents a collection of functionally characterized NFAT, AP-1, and GATA-3 binding sites. Element position is indicated in reference to the beginning of the 5′UTR of the gene. AP-1 and NFAT binding sites were independently identified using TRANSFAC (0.75/0.8) and compared with the published sequences. Distance between adjacent GATA-3 sites was measured between beginning positions of the cores. Base pairs (bp).

[ii] TFBS below default parameters. Percent identity (%ID) for each aligned TFBS (21 bp) was determined by rVISTA (n/a) not applicable. (1) Szabo et al. (1993); (2) Takemoto et al. (1997); (3) Li-Weber et al. (1994); (4) Lee et al. (1995); (5) Thomas et al. (1999); (6) Prieschl et al. (1995); (7) Stranick et al. (1997); (8)Cockerill et al. (1995); (9) Jenkins et al. (1995); (10) Masuda et al. (1993); (11) Gottshalk et al. (1993); (12) De Boer et al. (1999); (13)Kel et al. (1999); (14) Cakouros et al. (2001); (15) Rooney et al. (1995); (16) Burke et al. (2000); (17) Zheng and Flavell (1997); (18)Lee et al. (1998).