Table 1.

The Genomic Regions of PVA, Their Sizes, and the Numbers of Insertions Detected in Them

Region Size (nt)[iii] No. of insertions[ii]
Total Essential sites Nonessential sites
5′NTR16122202
P1891904446
HC-Pro13741526092
P3104113533102
6K11561349
CI190521860158
6K215920317
VPg567632043
NIa-Pro729911180
NIb154818839149
CP8071023171
3′NTR22731427
Total95651125329796

[i] 5′-NTR and 3′NTR: 5′- and 3′-nontranslated regions, respectively; P1, the first protein; HC-Pro, helper component-proteinase; P3, the third protein; 6K1 and 6K2, 6 kDa proteins 1 and 2; CI, cylindrical inclusion protein; VPg, viral genome-linked protein; NIa-Pro, proteinase; NIb, replicase; CP, coat protein.

[ii] Essential sites are those in which insertions prevented virus propagation and/or production of mature virions in protoplasts. Distribution of insertions within each of the genomic regions is shown in Fig.7.

[iii] Size in nucleotides (nt). For positions in the genome, see Fig.7.