Table 1.

Methanosarcina acetivoransGenome Features

General
Size5,751,492 bp
% G + C42.7%
Protein-coding genes4524
RNA genes68
Pseudogenes117
Percent coding74%
Average gene size936 bp (312 aa)
Average intergenic space size340 bp
Predicted protein-coding sequences
Identified by similarity to known sequences2226 (49%)
Conserved hypothetical proteins908 (20%)
Predicted proteins (no similarity to known sequences)1390 (31%)
Multigene families
Number of multigene families539
Number of genes in multigene families2178 (48%)
Domain comparison Genes with top BLASTX hit to domain Genes with BLASTX hits only to domain
Archaea1852 (41%)759 (17%)
Bacteria945 (21%)387 (9%)
Eucarya98 (2%)47 (1%)
Organism comparison Genes w/ BLAST hits Genes w/ top BLASTX hits Genes w/ BBH[i] hits Conserved clusters[ii]
Archaeoglobus fulgidus 1618 (36%)549 (12%)967 (21%)93 (256 genes)
Methanococcus thermoautotrophicum 1485 (33%)377 (8%)897 (20%)90 (295 genes)
Methanococcus jannaschii 1331 (29%)237 (5%)822 (18%)70 (194 genes)
Pyrococcus abyssi 1193 (26%)116 (3%)730 (16%)58 (151 genes)
Pyrococcus horikoshii 1141 (25%)96 (2%)690 (15%)47 (117 genes)
Halobacterium sp NRC-11067 (24%)125 (3%)731 (16%)65 (195 genes)

[i] Best bidirectional hit: Defined between two species as a pair of proteins, each of which has its top best BLASTPhit to the other when all proteins from both species are compared to one another.

[ii] A cluster in one species was defined as a set of genes with each member in the set no further than 10 genes from at least 1 other gene in the set. A conserved cluster was defined as a cluster inM. acetivorans with each member of the cluster having a best bidirectional hit (BBH) with a member of a single cluster in a second species. Thus, a conserved cluster consists of a set of genes “close” in the M. acetivorans genome whose BBH genes are also “close” in the genome of the second species. The number of genes indicates the number of genes in conserved clusters.