Two-Step RT-PCR for Quantification of Gene Expression in Varying Numbers of CD4+ Cells from Bronchoalveolar Lavage
| Number of CD4+cells | Ct | dCt | |||
| IL8 | IL13 | GAPDH | IL8/GAPDH | IL13/GAPDH | |
| 4 | 16.71 | 19.99 | 20.18 | 3.47 | 0.19 |
| 16 | 14.32 | 17.91 | 18.33 | 4.01 | 0.42 |
| 64 | 13.18 | 16.83 | 17.47 | 4.29 | 0.64 |
| 128 | 12.05 | 16.07 | 16.72 | 4.67 | 0.65 |