Molecular Analysis by Southern Blot Hybridization and PCR Amplification of the 2j Breakpoint Regions of the 30 2j Lines Used in This Study
| Name | Geographic origin | Hybridization bands (kb) | PCR products (kb) | ||||
| Proximal | Distal | B2-G6 | G5-D1 | R1-C2 | A1-C1 | ||
| j-1 | Carboneras (Spain) | 6.1 | 7.0 | 1.38 | 1.92 | 2.81 | 0.75 |
| j-2 | Carboneras (Spain) | 6.1 | 7.0 | 1.38 | 1.92 | 2.81 | 0.75 |
| j-3 | Carboneras (Spain) | 6.1 | 7.0 | 1.38 | 1.92 | 2.81 | 0.75 |
| j-4 | Carboneras (Spain) | 6.1 | 7.0 | 1.38 | 1.92 | 2.81 | 0.75 |
| j-5 | Carboneras (Spain) | 6.1 | 7.0 | 1.38 | 1.92 | 2.81 | 0.75 |
| j-6 | Carboneras (Spain) | 6.1 | 7.0 | 1.38 | 1.92 | 2.81 | 0.75 |
| j-7 | Caldetas (Spain) | 6.1 | 7.0 | 1.38 | 1.92 | 2.81 | 0.75 |
| j-8 | San Luis (Argentina) | 8.5 | 7.0 | 4.15 | 2.13 | 2.83 | 0.77 |
| j-9 | Quilmes (Argentina) | 5.0 | 8.5 | 1.32 | 2.07 | 4.34 | 2.28 |
| j-10 | Palo Labrado (Argentina) | 5.1 | 9.0 | 1.38 | 2.13 | — | — |
| j-11 | Los Negros (Bolivia) | 8.8 | 7.0 | 1.32 | 2.07 | 2.83 | 0.77 |
| j-12 | Guaritas (Brazil) | 8.8 | 7.0 | 1.32 | 2.07 | 2.83 | 0.77 |
| j-13 | Guaritas (Brazil) | 8.8 | 7.0 | 1.32 | 2.07 | 2.81 | 0.75 |
| j-14 | Laboratory (Australia) | 6.1 | 7.0 | 1.38 | 1.92 | 2.83 | 0.77 |
| j-15 | Catamarca (Argentina) | 6.1 | 7.0 | 1.38 | 1.92 | 2.81 | 0.75 |
| j-16 | Salta (Argentina) | 12.1 | 7.0 | 1.38 | — | 2.83 | 0.77 |
| j-17 | Tilcara (Argentina) | 6.0 | 7.0 | 1.38 | — | 2.83 | 0.77 |
| j-18 | Termas Rio Hondo (Argentina) | 5.0 | 7.0 | 1.32 | 2.07 | 2.83 | 0.77 |
| j-19 | Ticucho (Argentina) | 10.3 | 8.9 | 1.32 | 2.25 | — | — |
| j-20 | Hemmant Australia) | 6.1 | 7.0 | 1.38 | 1.92 | 2.81 | 0.75 |
| j-21 | Hemmant (Australia) | 6.1 | 7.0 | 1.38 | 1.92 | 2.81 | 0.75 |
| j-22 | Trinkey (Australia) | 8.8 | 7.0 | 1.32 | 2.07 | 2.83 | 0.77 |
| jz3-1 | Carboneras (Spain) | 9.9 | 7.0 | 1.32 | 3.11 | 2.83 | 0.77 |
| jz3-2 | Carboneras (Spain) | 9.9 | 7.0 | 1.32 | 3.11 | 2.81 | 0.75 |
| jz3-3 | Kariouan (Tunisia) | 9.9 | 7.0 | 1.32 | 3.11 | 2.83 | 0.77 |
| jz3-4 | Tilcara (Argentina) | 8.3 | 9.2 | 1.34 | — | — | — |
| jq7-1 | Carboneras (Spain) | 7.5 | 7.0 | 1.36 | — | 2.81 | 0.75 |
| jq7-2 | Mogan, Canary Islands (Spain) | 7.5 | 11.0 | 1.36 | — | 3.62 | 1.56 |
| jq7-3 | Caldetas (Spain) | 7.5 | 7.0 | 1.36 | — | 2.81 | 0.75 |
| jq7-4 | Otamendi (Argentina) | 6.1 | 7.0 | 1.38 | 1.92 | 2.83 | 0.77 |
[i] Hybridization bands are those obtained by Southern hybridization of PstI-digested genomic DNA of each line with theCD probe. Proximal and distal refer to the proximal and distal breakpoint, respectively. Proximal breakpoint bands indicated in boldface include a 3.8-kb extra segment due to a polymorphism in aPstI site. Products of each PCR were digested with different restriction enzymes: B2-G6, BamHI–EcoRI; G5-D1, R1-C2, and A1-C1, DraI.