Table 1.

Use of Autofinish on University of Washington Projects during 1998 and 1999

ProjectNo. of finishing readsNo. of Autofinish reads (% Autofinish reads)
djs201267161 (60%)
rg391h05390236 (61%)
djs10414090 (64%)
djs259148103 (70%)
PAO116371195 (73%)
djs333141112 (79%)
djs1198266 (80%)
djs228128104 (81%)
djs3629982 (83%)
djs146141121 (86%)
djs94127111 (87%)
djs327173156 (90%)
djs77113107 (95%)
djs306192183 (95%)
djs124149145 (97%)
djs301224219 (98%)
djs149191 (100%)
djs401111 (100%)
djs456666 (100%)
djs587272 (100%)
djs2928686 (100%)

[i] Autofinish (versions 5.0 to 9.0) was used on these projects at the University of Washington during 1998 and 1999. All are 150-kb to 200-kb BACs except for PA01, the six-megabase Pseudomonas aeruginosa bacterial genome. Immediately after shotgun, Autofinish was run for three rounds on each project: Each time, the lab made the reads suggested by Autofinish (without adding or removing any suggested reads) and reassembled the project. After this, if the project was still not finished, a human finisher manually completed the project. All of these projects have been submitted to GenBank as Phase 3 Complete sequence. (No. of finishing reads) The sum of Autofinish-selected reads and human-selected reads. (No. of Autofinish reads [% Autofinish Reads] The number and percentage of finishing reads that were suggested by Autofinish.