Nomenclature
| Symbol | Meaning |
| b | number of bases from one cut site to the next in a uniformly distributed library |
| i | number of sequenced clones |
| e | Euler's constant (≈2.71828) |
| m | user-specified level of restriction site bias |
| n | number of expected restriction sites per specified segment length |
| N | total number of clones in a multi-fold library |
| s | restriction enzyme specificity (number of bases) |
| χ | distance in bases along linearly arranged genome |
| θ exp | experimentally determined average overlap of sequenced clones (%) |
| G | linear genome length in bases (∼3 Gb for human genome) |
| L 0 | nominal clone length in bases (∼170,000 for BAC clones) |
| θ0 | required threshold to detect clone overlap in a mapping project (%) |
| L(i) | effective bases of genome covered after sequencing iclones |
| C(i) | effective percentage of genome covered after i clones =L(i)/G |
| θ(i) | redundancy after sequencing iclones |
| f n | Poisson probability density function for restriction site distribution |
| R(i) | rate of progress after sequencing i clones |
| ϕ | library depth |
| μ avg | Poisson average number of restriction sites per specified segment length |