Uneven Background Features
| CWRS-10 VDA design | |||||
| Forward | Average intensity | Standard deviation | Reverse | Average intensity | Standard deviation |
| A | 1510.5 | 164.6 | T | 2220.5 | 142.6 |
| C | 1209.0 | 99.1 | G | 1115.0 | 65.8 |
| G | 1263.0 | 94.9 | C | 1105.3 | 107.5 |
| T | 1724.8 | 96.4 | A | 2360.5 | 148 |
| CWRS-50 VDA design | |||||
| Forward | Average intensity | Standard deviation | Reverse | Average intensity | Standard deviation |
| A | 2575.0 | 225.9 | T | 4889.5 | 345.3 |
| C | 1548.0 | 176.2 | G | 3412.0 | 509.5 |
| G | 2856.0 | 225.0 | C | 7411.5 | 370.6 |
| T | 4278.5 | 460.9 | A | 5680.0 | 389.2 |
[i] Exemplar data from two different sites. CWRS-10 is from an X-linked region (FMR1) and the sample is male. Hence, the sample is haploid and cannot be a heterozygote. Nevertheless, it appears to be an A-T heterozygote. CWRS-50 is from an autosomal region (RET) and this particular sample is known to be a G-T heterozygote from previous genotyping (Carrasquillo, in prep.). By the criteria described in sections 1–3 (even background), the CWRS-10 site would be called a heterozygote, and the CWRS-50 site would be called N.