Transformation Efficiency of pYEXTSH3 and pYEXT2HIS3
| Vector construct | Concentration of CuSO4 | 0.3 mM | 0.5 mM | ||||
| w/o CuSO4 | 0.05 mM | 0.1 mM | |||||
| pYEXT2HIS3 | 1.2 × 103 | 1.2 × 103 | 2 × 104 | 4.8 × 103 | 1.2 × 103 | ||
| (A) pYEXTSH3 | 0 | 0 | 60 | 2.7 × 102 | 0 | ||
| (B) pYEXTGH3 (RF+) | 7 × 102 | 4 × 102 | 5 × 104 | 2.8 × 104 | 2 × 102 | ||
| (C) pYEXTGnH3 (RF−) | 10 | 3 × 102 | 10 | 0 | |||
[i] Transformation efficiency of pYEXTSH3 constructs (A–C) and the control plasmid pYEXT2HIS3 in Saccharomyces cerevisiaeGRF18 is shown to be dependent on concentration of CuSO4. The efficiencies are given in cfu/μg DNA.
[ii] (RF+), insert in frame with HIS3.
[iii] (RF−), insert not in frame with HIS3.