Summary of SAGE Libraries
| SAGE library | |||
| Forelimb | Hindlimb | ||
| Total SAGE tags excluding linkers[i] | 68,406 | 68,450 | |
| Unique transcripts | 23,140 | 22,329 | |
| Transcripts observed in both libraries | 9,169 | ||
| Linker contamination | 450 (0.65%) | 405 (0.64%) | |
| Frequency of duplicate DiTags[ii] | 1.05% | 0.84% | |
| Average GC content[iii] | 48.3 | 47.5 | |
| Number of clones sequenced | 3,639 | ||
| Averaged phredscore per base | 38 | 38 | |
[i] Linker sequences removed from our SAGE libraries were TCCCTATTAA and TCCCCGTACA.
[ii] Upon examination of several duplicate DiTags in the limb SAGE libraries, all were found to be composed of high abundance SAGE Tags (data not shown), indicating that the duplicate DiTags in these SAGE libraries were not due to DiTag amplification bias.
[iii] For an explanation of this value, in the context of the quality of a SAGE library, see Margulies et al. (2001).