Table 1.

Summary of Female Sterile Mutants

Genes required for patterning in the germarium
Locus Map position Alleles Notes
brainiac (brn) 4B2 brn198 Both alleles are hemizygous lethal, paternally rescued maternal
brn228 effect lethal over brnfs107. brn228/brn228 is a temperature sensitive lethal.
fs(1)Yb 3B1 fs(Yb)72 See text
ovarian tumor (otu) 7E9 otu55 otu67 otu139 otu55 and otu99 produce ovarian tumors (ONC class; Lindsley and Zimm 1992), out67 produces later phenotypes typical of the DIF class.
sans filles (snf) 4F2 snf148 Tumorous germarium
sex lethal (Sxl) 6F5 Sxlf79 Female lethal in hemizygotes. Homozygotes have tumorous ovaries.
fs(1)100 lethalAssociated with semi-lethality. 5% supernumary nurse cells (5 ring canals on oocyte).
fs(1)124 9D1–2See text
fs(1)217 26cM ± 2.5See text
fs(1)259 14B1-16A7See text
Genes required in mid-oogenesis
Locus Map position Alleles Notes
dunce (dnc) 3D2 dnc221b See text
dnc225
fs(1)K10 2F1 fs(1)K1047
fs(1)K10130
singed 7D2 sn77sn184
fs(1)3 5C5-5D1?Weak phenotype over deficiency, see text•
fs(1)140 43cM ± 2.5See text
fs(1)186 14C-15A6
fs(1)234 5A8-5C2See text
Mutations resulting in cell death or degeneration
Locus Map position Alleles Notes
fs(1)56 lethalVariable germ-line degeneration after stage 9. Few eggs, some short or fused dorsal appendages.
fs(1)60 0-2cM ± 1.4Variable germ-line degeneration after stage 10. No eggs laid.
fs(1)127 1cM ± 1Degeneration in stage 9 or 10, sometimes earlier (stage 6). Oocyte growth retarded after stage 9, nurse cell nuclei also small.Some tumorous cysts
fs(1)137a lethalAssociated with semi-lethality. Degeneration of germ-line cells usually at stage 9 but at variable stages starting in the germarium.
fs(1)162 9cM ± 2.7Variable degeneration after stage 9, majority normal to stage 14. No eggs laid.
fs(1)164 30cM ± 3.4See text
fs(1)192 24cM ± 1.6Variable degeneration after stage 10. Rare cases of 8− or (approx.) 32− cell cysts. Most appear normal to stage 14. Females lay rare collapsed egg remnants.
fs(1)221a 15-16cM ± 2.4See text
fs(1)242 lethalVariable degeneration after stage 9. Females lay eggs with fused or reduced dorsal appendages.
fs(1)250 21cM ± 1Degenerate after stage 12. Females lay small number of collapsed egg remnants.
fs(1)260 [ii] Degeneration in stage 10 or later. Rare failed or retarded border cell migration. Rare supernumerary nurse cells (<5%).
fs(1)261EL lethalDegeneration after stage 8. Associated with semi-lethality.
Genes reguired for egg shell formation
Locus Map position Alleles Notes
dec-1 7C6 dec-1H12 All alleles result in few or no eggs laid
dec-1H13
dec-1H15
fs(1)38 25cM ± 2.3 fs(1)38 Few eggs, defective chorion
fs(1)161

[i] Cytological map intervals denote limits of deficiencies that uncover the mutation; “lethal” indicates the presence of a lethal mutation on the chromosome (in the fslocus or outside of it) that prevents recombination mapping.

[ii] Mapping revealed a contamination problem.