Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) Results and Primer Sequences
| ORFs | Amplicon size (bp) | RT-PCR | Sense primer | Antisense primer | |||||
| S.P. | E.P. | ||||||||
| B0220 | 381 | + | + | 45U21: GACAGTCGCCGCTCTGGTCAT | 405L21: GCCGCGAACAACACCGTTTTA | ||||
| B0279 | 481 | − | − | 305U21: GCATCAATCGAAGCCGAGGTA | 766L20: TCTGTTTTGCGGAGCACTGG | ||||
| B0358 | 445 | + | + | 17U21: TATTAGCGGCGGCCATTTTTA | 441L21: GTGATCACCGGGCCAGTATAG | ||||
| B0364 | 270 | + | + | 14U21: CCGGCAACTCTGGCACAGATG | 263L21: TTCGGCAGGCAACCATTGAAG | ||||
| B0558 | 276 | + | + | 75U20: CGATCAAGTGCGAAAAGATT | 332L19: CATCGGAAAACTCCTGCTT | ||||
| B0645 | 438 | − | + | 244U21: TGTGAATGGCGTGACTATCTT | 661L21: TTCCGGGCATGCAGAATAGTA | ||||
| B0648 | 620 | + | + | 66U21: TTGCAAACCCGCGCATGACTC | 664L22: GCGTTACCGGGCATTCAGACTG | ||||
| B0836 | 234 | + | + | 107U21: TCAGCGAAAGCAATCATCTAC | 320L21: TCAGCGTTAAGCTCATCAGAC | ||||
| B0853 | 376 | + | + | 46U21: CTCGATGACCTGGGGATGAGT | 401L21: ACAGCAGTTGATGGGCATTAG | ||||
| B1085 | 282 | + | + | 5U18: CTAGTCGCGTCGCCAACC | 270L17: AGGCCGTTTCCGTGTCC | ||||
| B1143 | 292 | + | + | 84U20: CACAGCCGAACAGACCAAAA | 356L20: CTTGATGGGCTTAGGCGTAA | ||||
| B1155 | 346 | + | + | 60U21: TCCGCCAGATTCTAAAGAAAT | 385L21: CTGAACTGCCGGAGGCGTAGG | ||||
| B1549 | 165 | + | + | 182U21: TAAAGGGCCTAAAAAACATTG | 326L21: TTTGTTCGTGGATATTTGTAA | ||||
| B1668 | 502 | + | − | 966U21: ACAACGCGACCCATTTGACTG | 1449L19: CGCTACGCGCCCACGAATA | ||||
| B1751 | 792 | + | + | 46U20: GCCTGGCGCTGTTTATTCTC | 818L20: TTCGGCGATTTTAAAGGTAA | ||||
| B1972 | 367 | + | + | 165U20: GCTGGCGACCTTGTTAATCA | 512L20: GAGCGGCTGCGGTGAAATAA | ||||
| B2420 | 327 | + | + | 394U21: AAAGAGCCGGATCTGGACTGT | 700L21: AAACGCCGTAAGGTCATCAAT | ||||
| B2625 | 410 | − | − | 634U21: AGGAAAAACGAGCCCGAGAAA | 1023L21: GCCATGTTCTCCGCCATTTTA | ||||
| B2626 | 536 | + | + | 64U21: ATTGATGACTCCCTAAACGAA | 581L19: TCTCCGTCACCGACTATTT | ||||
| B2630 | 834 | − | − | 188U20: ACGGGGACGGTTCGACTACA | 1000L22: TCAACCGGGCCATATCAGAAAT | ||||
| B2760 | 1123 | − | − | 114U21: GCCCCGTGACGATATGGAACT | 1214L23: TCCGGCCCCTTTGAAGTCTTTAT | ||||
| B2847 S.P. | 331 | + | N.A. | 66U23: TAACGCCGATCTTAACGTCGTTC | 642L23: TCCCCGAATCCAGTATCCGTGTT | ||||
| B2847 E.P. | 294 | N.A. | + | 371U23: ATGCCCCACCTGCCATAAAAGTT | 642L23: TCCCCGAATCCAGTATCCGTGTT | ||||
| B3618 | 705 | + | + | 141U21: TCTGGCCGCACTTGAAAATGA | 825L21: GCCTGCATTTTTGCCCCTAAA | ||||
| B3875 | 324 | − | − | 99U21: CTCCGATCAGGGCGAGGTTAT | 402L21: TCCAGTTCCCCGCTCAAATCT | ||||
| B4215 | 455 | − | − | 33U23: TGGTGCGGTGGAGTTATTTGTAA | 465L23: CCAATCGCCTGGTGAATATAACC | ||||
[i] (ORF) Open reading frame; (E.P.) exponential phase; (S.P.) stationary phase.