Markup | Genome Research

Table 1.

Intron/Exon Boundaries of the DGLR-2 Gene

Intron	5′ Donor	Intron size (bp)	3′ Acceptor	Intron phase
1 (variant1)	TTT gtaaggt....	∼7 kb	....cccttag ACA	–
1 (variant2)	AAG gtgccac....	∼7 kb	....cccttag ACA	–
1 (variant3)	TTG gtgccac....	∼7 kb	....cccttag ACA	–
1 (variant4)	TTT gtaaggt....	∼7 kb	....taaatag ATA	–
2	CT gtgagta....	1223 bp	....ccaacag A	2
	Leu		Leu
3	CT gtgagta....	583 bp	....tttgcag A	2
	Leu		Leu
4	CT gtaagta....	325 bp	....cacacag A	2
	Leu		Leu
5	CT gtgagcc....	591 bp	....tcgacag C	2
	Leu		Leu
6	CT gtaagta....	74 bp	....tttgcag T	2
	Leu		Leu
7	TT gtaagta....	1747 bp	....tttgcag A	2
	Leu		Leu
8	CT gtaagta....	1083 bp	....ccttcag T	2
	Leu		Leu
9	CT gtgagta....	55 bp	....ttttcag G	2
	Leu		Leu
10	TT gtgagtt....	65 bp	....actgcag A	2
	Leu		Leu
11	TT gtaagtt....	57 bp	....cacccag A	2
	Leu		Leu
12	CT gtgcgtt....	61 bp	....ttcacag T	2
	Leu		Leu
13	C gtaagtg....	56 bp	....tccccag AT	1
	His		His
14	G gtaagtg....	57 bp	....tgtacag CG	1
	Ala		Ala

[i] The sequence of each of the intron-exon boundaries is shown, as well as the codons for the amino acid residues. Uppercase and lowercase letters represent nucleotides in the exons and introns, respectively. For intron 1, the four splicing variants that we have isolated are given. These variants differ from each other, depending on which 5′ donor and 3′ acceptor sites are used. The sequences of the introns are given in our GenBank/EMBL Database submission. The overall positions of the introns are shown in Figs. 1 and 2. Introns 9-12 occur at the same positions within the gene and have the same intron phasing as four introns in the genes of the mammalian glycoprotein hormone receptors (• in Fig. 3).