Most Frequently Used Metabolites in E. coliCentral Metabolism
| Occurrence | Name of metabolite |
| 205 | H2O |
| 152 | ATP |
| 101 | ADP |
| 100 | phosphate |
| 89 | pyrophosphate |
| 66 | NAD |
| 60 | NADH |
| 54 | CO2 |
| 53 | H+ |
| 49 | AMP |
| 48 | NH3 |
| 48 | NADP |
| 45 | NADPH |
| 44 | Coenzyme A |
| 43 | l-glutamate |
| 41 | pyruvate |
| 29 | acetyl-CoA |
| 26 | O2 |
| 24 | 2-oxoglutarate |
| 23 | S-adenosyl-l-methionine |
| 18 | S-adenosyl-homocysteine |
| 16 | l-aspartate |
| 16 | l-glutamine |
| 15 | H2O2 |
| 14 | glucose |
| 13 | glyceraldehyde-3-phosphate |
| 13 | THF |
| 13 | acetate |
| 12 | PRPP |
| 12 | [acyl carrier protein] |
| 12 | oxaloacetic acid |
| 11 | dihydroxy-acetone-phosphate |
| 11 | GDP |
| 11 | glucose-1-phosphate |
| 11 | UMP |
| 10 | e− |
| 10 | phosphoenolpyruvate |
| 10 | acceptor |
| 10 | reduced acceptor |
| 10 | GTP |
| 10 | l-serine |
| 10 | fructose-6-phosphate |
| 9 | l-cysteine |
| 9 | reduced thioredoxin |
| 9 | oxidized thioredoxin |
| 9 | reduced glutathione |
| 8 | acyl-ACP |
| 8 | l-glycine |
| 8 | GMP |
| 8 | formate |
[i] Metabolites were used either as reactants or products.