Invader Assay Design Summary
| Locus | Design no.[i] | Signal arm[iii] | FRET probe[iv] | Secondary oligo[v] | Annealing temp.(°C)[v] | |
| Failed initial QC | Passed initial QC | |||||
| INSg.-23A>T | 1 (S)e | 1 | 1 | 1 | 66 | |
| INSg.1127C>T | 1 (S) | 2 | 1 | 1 | 68 | |
| LRP5g.-14279A>G | 1 (S) | 1 | 1 | 1 | 60 | |
| LRP5g.-11215T>A | 1 (AS) | 2 (S) | 1 | 1 | 1 | 71 |
| LRP5g.-11094G>A | 1 (AS) | 2 (S) | 3 | 2 | 4 | 66 |
| LRP5g.-10088G>A | 1 (S) | 1 | 1 | 1 | 68 | |
| LRP5g.-9693G>A | 1 (AS) | 1 | 1 | 1 | 68 | |
| LRP5g.-5802G>C | 1 (AS) | 3 | 2 | 4 | 68 | |
| LRP5g.-5677C>T | 1 (AS) | 3 | 1 | 2 | 66 | |
| LRP5g.-5264G>A | 1 (AS) | 1 | 1 | 1 | 66 | |
| LRP5g.-864A>G | 1 (AS) | 1 | 1 | 1 | 63 | |
| LRP5g.2221C>T | 1 (AS) | 1 | 1 | 1 | 65 | |
| LRP5g.3103C>G | 1 (AS) | 2 | 2 | 3 | 66 | |
| LRP5g.4780G>C | 1 (AS) | 4 | 2 | 5 | 66 | |
| LRP5g.5257T>G | 1 (AS) | 2 (S) | 1 | 2 | 3 | 63 |
| LRP5g.7374G>A | 1 (AS) | 1 | 1 | 1 | 65 | |
| LRP5g.13963C>T | 1 (S) | 4 | 1 | 2 | 64 | |
| LRP5g.24964C>T | 1 (S) | 2 | 1 | 1 | 66 | |
| LRP5g.28149C>T | 1 (S) | 2 | 1 | 1 | 65 | |
| LRP5g.31856G>A | 1 (AS) | 3 | 1 | 2 | 71 | |
| LRP5g.35592T>C | 1 (AS) | 2 | 1 | 1 | 65 | |
| LRP5g.42125G>T | 1 (S) | 2 | 2 | 3 | 66 | |
| LRP5g.45704G>A | 1 (AS) | 2 | 1 | 1 | 68 | |
| EST3c.448G>A | 1 (AS) | 2 (S) | 2 | 1 | 1 | 69 |
| EST4c.3021–3026del | 1 (AS), 2 (S) | 3 (S) | 1 | 1 | 2 | 63 |
| EST4c.3211G>A | 1 (AS) | 3 | 1 | 2 | 68 | |
| EST4c.3403G>A | 1 (AS) | 2 | 1 | 1 | 62 | |
| IL4-Rc.1216T>C | 1 (S) | 2 | 1 | 1 | 66 | |
| IL4-Rc.1902A>G | 1 (AS) | 4 | 1 | 2 | 68 | |
| GCG-R G40S | 1 (S) | 2 | 1 | 1 | 65 | |
| ICAM-1 G241R | 1 (AS) | 2 | 1 | 1 | 68 | |
| ICAM-1 K469E | 1 (AS) | 4 | 1 | 2 | 68 | |
| CTLA4g.-651C>T | 1 (S) | 4 | 1 | 2 | 66 | |
| CTLA4g.49A>G | 1 (AS) | 2 (AS) | 2 | 1 | 1 | 66 |
| CTLA4g.920C>T | 1 (S) | 2 | 1 | 1 | 62 | |
| CTLA4g.-318C>T | 1 (S) | 2 (S) | 2 | 1 | 1 | 64 |
| IRS1 G972R | 1 (AS) | 2 | 1 | 1 | 68 | |
[i] Initial quality control is defined in Methods.
[ii] (S) Sense strand; (AS) antisense strand.
[iii] Number of oligonucleotides in the signal arm.
[iv] FRET probe.
[v] Secondary target and the primary annealing temperature used for each assay.